Anti-Mouse CD23 (2G8) ColorWheel® Dye-Ready mAb CWA-1003-25UL

Specificity
Clone 2G8 is a rat monoclonal antibody that detects murine CD23.
FceR isolated from murine B cell lymphoma cell line M12.4.5
Quality Control Testing:
Evaluated by Flow Cytometry in Mouse splenocytes.
Flow Cytometry Analysis: Staining of one million mouse splenocytes was performed using 5 µL of 1:1 mixture of Cat. No. CWA-1003, Anti-Human CD23 (2G8) Colorwheel® Dye-Ready mAb and CWD-PE, Colorwheel® Antibody-Ready Phycoerythrin (PE) Dye or an equivalent amount of a PE-conjugated Rat IgG2a isotype control.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-Mouse CD23 (2G8) ColorWheel<® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any Colorwheel® antibody with any Colorwheel® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each Colorwheel® antibody and dye is lyophilized for long long-term storage, allowing for simplicity and flexibility without compromise
Target description
Low affinity immunoglobulin epsilon Fc receptor (UniProt: P20693; also known as Fc-epsilon-RII, Lymphocyte IgE receptor, CD23) is encoded by the Fcer2 (also known as Fcr2a) gene (Gene ID: 14128) in murine species. CD23 is a homotrimeric, single-pass type II membrane glycoprotein that contains a cytoplasmic domain (aa 1-23); a transmembrane domain (aa 24-49), and an extracellular domain (aa 50-331). The extracellular domain presents striking similarity to C-type lectins. CD23 expression is reported in B cells, monocyte, follicular dendritic cells, platelets, and eosinophils. It is also expressed in cases of chronic lymphocytic leukemia (CLL) and in follicular lymphomas. It serves as a low-affinity receptor for immunoglobulin E (IgE) and CR2/CD21 and plays an essential role in the regulation of IgE production and in B-cell differentiation. In addition to its role as a receptor for IgE, CD23 can also be released from cell surface as a soluble protein (sCD23) via ADAM10-mediated ectoderm shedding. Mice lacking ADAM10 expression in their B cells display defective release of CD23. Clone 2G8 is reported to interact at a site similar to but not identical with the site on CD23 that binds IgE. (Ref.: Kintner, C., and Sugden, B. (1981). Nature. 294(3); 458-460; Margaret, E., et al. (1999). J. Immunol. 163(1); 217-223; Acharya, M., et al. (2010). Clin. Exp. Immunol. 162(1); 12-23).
Physical form
Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted antibody solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.
Reconstitution
1.0 mg/mL after reconstitution at 25 μL in PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL of PBS. Protect from light.
ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany

Quality Level	200
biological source	rat
antibody form	purified antibody
antibody product type	primary antibodies
clone	2G8, monoclonal
product line	ColorWheel®
form	lyophilized
mol wt	calculated mol wt 37.65 kDa
species reactivity	mouse
packaging	antibody small pack of 25 µL
application(s)	flow cytometry: suitable
isotype	IgG2b
epitope sequence	Unknown
Protein ID accession no.	NP_001240672.1,
UniProt accession no.	P20693,
shipped in	ambient
storage temp.	2-8°C
Gene Information	mouse ... Fcer2(14128)