Anti-Mouse CD11b (M1/70) ColorWheel® Dye-Ready mAb CWA-1006-25UL

Specificity
Clone M1/70 is a rat monoclonal antibody that detects murine CD11b.
Mouse spleen cells.
Quality Control Testing:
Evaluated by Flow Cytometry in mouse bone marrow cells.
Flow Cytometry Analysis (FC): Staining of one million mouse bone marrow cells was performed using 5 µL of a 1:1 mixture of Cat. No. CWA-1006, Anti-CD11b Antibody (M1/70) Colorwheel® Rat mAb and Cat. No. CWD-PE Colorwheel® activated Phycoerythrin (PE) Dye or an equivalent amount of PE-conjugated Rat IgG2b isotype control.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-Mouse CD11b (M1/70) ColorWheel<® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any Colorwheel® antibody with any Colorwheel® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each Colorwheel® antibody and dye is lyophilized for long long-term storage, allowing for simplicity and flexibility without compromise
Target description
Integrin alpha-M (UniProt: P05555; also known as CD11 antigen-like family member B, CR-3 alpha chain, Cell surface glycoprotein MAC-1 subunit alpha, Leukocyte adhesion receptor MO1, CD11b) is encoded by the Itgam gene (Gene ID: 16409) in murine species. Integrins are alpha/beta heterodimeric adhesion receptors that couple the extracellular matrix on other cells with the contractile cytoskeleton to transduce mechanochemical signals across the plasma membrane. CD11b is a single-pass type I membrane protein that is synthesized with a signal peptide (aa 1-16), which is cleaved off in the mature form that contains a long extracellular domain (aa 17-1105), a transmembrane domain (aa 1106-1129), and a short cytoplasmic domain (aa 1130-1153). It is predominantly expressed in monocytes and granulocytes. It is usually found as a heterodimer of alpha and beta subunits and associates with Integrin beta-2 (ITGB2). It has been implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It contains a metal ion-dependent adhesion site (MIDAS) in the integrin A domain, which is essential for stabilization of domain A in high affinity state. The major ligand recognition site of CD11b/CD18 has been mapped to the A-domain in the CD11b subunit (CD11bA). Both magnesium and calcium ions exhibit equal affinities for the inactive CD11bA, however, upon activation there is a 10-fold increase in the binding affinity of magnesium ions without affecting affinity of calcium ions. Two isoforms of CD11b have been reported that are produced by alternative splicing.
Physical form
Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted antibody solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.
Reconstitution
1.0 mg/mL after reconstitution at 25 μL in PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL of PBS. Protect from light.
ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany

Quality Level	200
biological source	rat
antibody form	purified antibody
antibody product type	primary antibodies
clone	M1/70, monoclonal
product line	ColorWheel®
form	lyophilized
mol wt	calculated mol wt 127.48 kDa
species reactivity	mouse
species reactivity (predicted by homology)	human, rat
packaging	antibody small pack of 25 µL
application(s)	flow cytometry: suitable
isotype	IgG2b
epitope sequence	Unknown
Protein ID accession no.	ABK96806.1,
UniProt accession no.	P05555,
shipped in	ambient
storage temp.	2-8°C
Gene Information	mouse ... Itgam(16409)