Anti-mono- ADP-ribose binding reagent

eCl@ss:

32160702

Кат. номер

MABE1076-25UG

MABE1076

Anti-mono-ADP-ribose binding reagent is a His-tagged recombinant protein fused to rabbit Fc tag, expressed in and purified from Rosetta (DE3)pLysS strain of E. coli (Cat. No. 70956). It is useful for the affinity detection of both mono-ADP-ribosylated proteins on membranes in a manner similar to antibody-based Western and dot blot analysis. The rabbit Fc tag allows visualization of the binding with conjugated anti-rabbit secondary antibodies. The Fc tag also allows Anti-ADP-ribose binding reagent to be captured on Protein A resins for affinity pull-down applications.
Specificity
This reagent binds to mono-ADP ribosylated proteins.
Western Blotting Analysis: 0.4 µg/mL of a representative lot detected mono-ADP-ribosylated proteins.
Dot Blot Analysis: A representative lot detected mono-,-ADP-ribose modified proteins.
Dot Blot Analysis: A representative lot detected mono-ADP ribosylated proteins. (Courtesy of Lee Kraus, University of Texas Southwestern Medical Center).
Western Blotting Analysis: A representative lot detected mono-ADP-ribose modified protein by Wester blotting (Courtesy of Lee Kraus, University of Texas Southwestern Medical Center).
Research Category
Epigenetics & Nuclear Function
Anti-mono-ADP-ribose binding reagent, Cat. No. MABE1076, is a reagent that targets mono (ADP-ribose) modified proteins and has been tested in Dot Blot and Western Blotting.
Quality
Evaluated by Gel Electrophoresis.
Gel Electrophoresis Analysis: 0.5 µg of this binding reagent was analyzed on GEL Electrophoresis to test for purity.
Target description
Variable depending on the target proteins and the extent of ADP-ribosylation.
Physical form
Format: Purified
Immobilized Metal Affinity Chromatography (IMAC)
Purified from E. coli and supplied in buffer containing 10 mM Tris pH 7.5, 0.2 M NaCl, 10 mM Imidazole, 1 mM PMSF, 1 mM beta-Mercaptoethanol, with 10% glycerol without preservatives.
Storage and Stability
Stable for 1 year at -80°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -80°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	Escherichia coli
Quality Level	100,
antibody form	affinity isolated antibody
antibody product type	primary antibodies
clone	monoclonal
species reactivity	mouse, human
packaging	antibody small pack of 25 µg
application(s)	dot blot: suitable, western blot: suitable
shipped in	ambient

WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable