Anti-MLH1 (N-terminal) antibody produced in rabbit

NACRES:

NA.41

Кат. номер

M8320-25UL

M8320-200UL

MutL homolog 1 (MLH1) is a nucleoprotein and a major component of mismatch repair system. The MLH1 gene is localized on chromosome 3p21 and is made up of 19 exons. The protein has a molecular weight of 80kDa.
MutL homolog 1 (MLH1) is part of a large multi-subunit protein complex of tumor suppressors, DNA damage sensors and signal transducers, named BRCA1-associated genome surveillance complex (BASC).
synthetic peptide corresponding to amino acids 60-75 of human MLH1, conjugated to KLH via an N-terminal added cysteine residue. The corresponding peptide sequence is conserved in human, rat, and mouse.

• Anti-MLH1 (N-terminal) antibody produced in rabbit has been used in:
• immunoblotting
• immunoprecipitation
• immunocytochemistry

Biochem/physiol Actions
MutL homolog 1 (MLH1) has been shown to be involved in stimulating carcinogenesis in the colon.
MutL protein (MLH), a homolog of the E. coli MutL gene, is involved in DNA mismatch repair. Nonpolyposis colorectal cancer-2 is caused by a hereditary mutation in the MLH1 gene. This cancer can also be a result of hypermethylation of one MLH1 allele in somatic cells (a germline epimutation).
Target description
MLH1 (N-terminal) is part of a large multi-subunit protein complex of tumor suppressors, DNA damage sensors, and signal transducers, named BASC (BRCA1-associated genome surveillance complex).
Physical form
Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit
Quality Level	200
antibody form	affinity isolated antibody
antibody product type	primary antibodies
clone	polyclonal
form	buffered aqueous solution
mol wt	antigen 80-85 kDa
species reactivity	mouse, human, rat
packaging	antibody small pack of 25 µL
concentration	~1 mg/mL
application(s)	immunocytochemistry: 2.5-5 µg/mL using MCF7 cells fixed with paraformaldehyde-Triton","immunoprecipitation (IP): 5-10 µg using Jurkat cell lysates","western blot: 0.5-1 µg/mL using Jurkat cell lysates
conjugate	unconjugated
UniProt accession no.	P40692,
shipped in	dry ice
storage temp.	20°C
Gene Information	human ... MLH1(4292) mouse ... Mlh1(17350) rat ... Mlh1(81685)

Personal Protective Equipment	Eyeshields,, Gloves,, multi-purpose combination respirator cartridge (US),
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable