Anti-Meis1/2

eCl@ss:	32160702
NACRES:	NA.41

Кат. номер

ABE2865-25UG

ABE2865-100UG

Homeobox protein Meis1 (UniProt: Q60954-1; also known as Myeloid ecotropic viral integration site 1) Homeobox protein Meis2 (UniProt: P97367-2; also known as Meis1-related protein 1) are encoded by the Meis1 and Meis2 (also known as Mrg1, Stra10) genes (Gene ID: 17268 and 17536) in murine species. Meis proteins act as transcription factors that are orthologous to the Drosophila homothorax (Hth) protein. They are expressed dynamically in several organs during embryogenesis and exert potent regulatory activity through their interaction with Hox proteins and other transcription factors. Meis protein contain a TALE (three-amino-acid loop extension) sub-class of the homeodomain that binds to DNA. They directly bind to Pbx proteins and the Meis/Pbx protein complex binds to DNA through respective Meis- and Pbx-consensus binding sites to regulate transcription. The Meis/Pbx complex plays important roles during development of multiple organs. In humans and mice, three homologues Meis1, Meis2 and Meis3 have been identified. Meis1 is expressed at high levels in the lung and at lower levels in the heart and brain. On the other hand, Meis 2 displays spatially restricted expression patterns in the developing nervous system, limbs, face, and in various viscera. In adult, it is mainly expressed in the brain and female genital tract, with a different distribution of the alternative splice forms in these organs. Pancreatic beta cells express both Meis1 and 2. Both Meis1 and 2 are expressed at high levels in all stages of embryonic development of mice (day 7 to 17). Meis3 is expressed in pancreatic islets and is reported to control the expression of expression of PDK1, which promotes survival of pancreatic beta-cells. Deficiency of Meis1 or Meis 2 can lead to embryonic lethality. Meis1-deficient mice die around midgestation due to a general failure of definitive hematopoiesis. (Ref.: Machon, O et al. (2015). BMC Dev. Biol. 515:40; Azcoitia, V et al., (2005). Dev. Biol. 280 (2); 307-320).
Specificity
This rabbit polyclonal antibody detects Meis1/2. It targets an epitope with in 14 amino acids from the C-terminal region.
Epitope: C-terminus
KLH-conjugated linear peptide corresponding to 14 amino acids from the C-terminal region of murine Meis1a, Meis2a, and 13 amino acids from Meis2b isoforms.
Immunofluorescence Analysis: A representative lot detected Meis1/2 in Immunofluorescence applications (Mercader, N., et. al. (2005). Development. 132(18):4131-42; Marcos, S., et. al. (2015). Development. 142(17):3009-20).
Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected Meis1/2 in Chromatin Immunoprecipitation applications (Penkov, D., et. al. (2013). Cell Rep. 3(4):1321-33; Marcos, S., et. al. (2015). Development. 142(17):3009-20).
Western Blotting Analysis: A representative lot detected Meis1/2 in Western Blotting applications (Mercader, N., et. al. (2005). Development. 132(18):4131-42).
Anti-Meis1/2 Antibody, Cat. No. ABE2865, is a highly specific rabbit polyclonal antibody that targets Meis1/2 and has been tested in Chromatin Immunoprecipitation (ChIP), Immunofluorescence, and Western Blotting.
Research Category
Epigenetics & Nuclear Function
Quality
Evaluated by Western Blotting in HEK293T cells transfected with pCMVTAG2-Flag-Meis2a.
Western Blotting Analysis: 2 µg/mL of this antibody detected Meis1/2 in HEK293T cells transfected with pCMVTAG2-Flag-Meis2a.
Target description
~51 kDa observed; 43.00, 43.79, and 43.06 kDa for Meis1a, Meis2a, and Meis2b, respectively. Uncharacterized bands may be observed in some lysate(s).
Physical form
Purified rabbit polyclonal antibody in PBS with 0.05% sodium azide and 50% glycerol.
Format: Purified
Protein A purified
Storage and Stability
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit
antibody form	purified antibody
antibody product type	primary antibodies
clone	polyclonal
species reactivity	mouse
species reactivity (predicted by homology)	human (based on 100% sequence homology), salamander (based on 100% sequence homology)
packaging	antibody small pack of 25 µg
application(s)	ChIP: suitable","immunofluorescence: suitable","western blot: suitable
NCBI accession no.	NP_001129544,","NP_001153039,","NP_001180200,
UniProt accession no.	P97367-2,","P97367-3,","Q60954-1,