Anti-Mediator complex subunit 25

eCl@ss:	32160702
NACRES:	NA.41

Кат. номер

ABE1420-25UG

ABE1420

Mediator of RNA polymerase II transcription subunit 25 (UniProt: Q71SY5; also known as Activator interaction domain-containing protein 1, Activator-recruited cofactor 92 kDa component, ARC92, Mediator complex subunit 25, p78) is encoded by the MED25 (also known as ACID1, ARC92, PTOV2, TCBAP0758) gene (Gene ID: 81857) in human. It is a member of the Mediator complex subunit 25 family that is ubiquitously expressed. Higher expression levels are observed in brain, heart, kidney, peripheral leukocytes, placenta, skeletal muscle and spleen. It acts as a coactivator that is involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator complex subunit 25 functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. It is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Six isoforms of Mediator complex subunit 25 have been described that are generated by alternative splicing. Defects in MED25 gene are known to cause Charcot-Marie-Tooth disease 2B2, a disorder of the peripheral nervous system characterized by progressive weakness and atrophy.
Specificity
This rabbit polyclonal antibody detects Mediator of RNA polymerase II transcription subunit 25 (MED25) in human and murine species. it targets an epitope within 16 amino acids from the internal region. Detects all isoforms of Mediator complex subunit 25.
KLH-conjugated linear peptide corresponding to 16 amino acids from the internal region of human Mediator of RNA polymerase II transcription subunit 25 (MED25).
Research Category
Epigenetics & Nuclear Function
Anti-Mediator complex subunit 25, Cat. No. ABE1420, is a highly specific rabbit polyclonal antibody that targets MED25 and has been tested in Immunohistochemistry (Paraffin), Peptide Inhibition Assay, and Western Blotting.
Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Mediator complex subunit 25 in human cerebellum and mouse cerebral cortex tissues.
Peptide Inhibition Analysis: 2 µg/mL from a representative lot blocked Mediator complex subunit 25 in mouse brain tissue lysate.
Quality
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: 2 µg/mL of this antibody detected Mediator complex subunit 25 in 10 µg of HeLa cell lysate.
Target description
~70 kda observed; 78.17 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physical form
Affinity Purified
Purified rabbit polyclonal antibody in buffer containing 0.1M Tris-Glycine, 0.15 M NaCl, pH 7.4 with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit
Quality Level	100
antibody form	affinity isolated antibody
antibody product type	primary antibodies
clone	polyclonal
purified by	affinity chromatography
species reactivity	human, mouse
species reactivity (predicted by homology)	bovine (based on 100% sequence homology), yeast (based on 100% sequence homology), rat (based on 100% sequence homology)
packaging	antibody small pack of 25 µg
application(s)	immunohistochemistry: suitable (paraffin)","inhibition assay: suitable (peptide)","western blot: suitable
NCBI accession no.	NP_112235.2,
UniProt accession no.	Q71SY5,
shipped in	ambient

Flash Point F	does not flash
Flash Point C	does not flash