Anti-LRIG1 Antibody, clone 2D1.1

eCl@ss:

32160702

Кат. номер

MABS1816

MABS1816-25UG

Leucine-rich repeats and immunoglobulin-like domains protein 1 (UniProt: Q96JA1; also known as LIG-1) is encoded by the LRIG1 (also known as LIG1) gene (Gene ID: 26018) in human. LIG-1 is a widely expressed single-pass type I membrane protein that acts as a feedback negative regulator of signaling by receptor tyrosine kinases, through a mechanism that involves enhancement of receptor ubiquitination and accelerated intracellular degradation. LIG-1 contains 3 Ig-like C2-type (immunoglobulin-like) domains, 15 LRR (leucine-rich) repeats, 1 1 LRRCT domain, and 1LRRNT domain. The LRRs and the Ig-domains are each sufficient for EGFR/ERBB1 binding and this interaction is abolished only when these two domains are deleted. LIG-1 is highly expressed by intestinal stem cells and controls the size of the intestinal stem-cell niche by regulating the amplitude of growth-factor signaling. It is also reported to control stem-cell proliferation in epidermis. LIG-1 is reported to be expressed in a gradient with highest levels in intestinal stem cells and it is absent from Paneth cells. About one-third of all cells in the intestinal crypts are shown to express LIG-1. (Ref.: Wong VWY., et al. (2012). Nat. Cell Biol. 14(4); 401-408).
Specificity
Clone 2D1.1 detects Leucine-rich repeats and immunoglobulin-like domains protein 1 (LIG-1) in human. It targets an epitope within 134 amino acids from the C-terminal end.
GST/His-tagged recombinant fragment corresponding to 134 amino acids from the C-terminal end of human Leucine-rich repeats and immunoglobulin-like domains protein 1 (LRIG1).
Anti-LRIG1 Antibody, clone 2D1.1, Cat. No. MABS1816, is a mouse monoclonal antibody that targets human Leucine-rich repeats and immunoglobulin-like domains protein 1 (LRIG1) and has been tested for use in Western Blotting.
Research Category
Signaling
Quality
Evaluated by Western Blotting in LNCaP cell lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected LRIG1 in 10 µg of LNCaP cell lysate.
Target description
~150 kDa observed; 119.11 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physical form
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G purified
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality Level	100
biological source	mouse
antibody form	purified immunoglobulin
antibody product type	primary antibodies
clone	2D1.1, monoclonal
species reactivity	human
packaging	antibody small pack of 25 µg
application(s)	western blot: suitable
isotype	IgG1
NCBI accession no.	NP_056356,
UniProt accession no.	Q96JA1,
shipped in	ambient
Gene Information	human ... LRIG1(26018)

WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable