Anti-Livin (C-terminal) antibody produced in rabbit

NACRES:

NA.41

Кат. номер

L9544-25UL

L9544-200UL

Livin or baculoviral IAP repeat-containing 7 (BIRC7) belongs to the family of the inhibitor of apoptosis proteins (IAP). It contains a single copy of a baculovirus IAP repeat (BIR) as well as a really interesting new gene (RING)-type zinc finger domain. Structurally, livin comprises a three-stranded anti-parallel β-sheet and four α-helices and takes a globular architecture. BIRC7 gene is mapped to human chromosome 20q13.33 and is expressed in α and β isoforms.
Specificity
Anti-Livin (C-terminal) recognizes human Livin/BIRC7.
Anti-Livin (C-terminal) antibody produced in rabbit has been used in immunoblotting and immunoprecipitation.
Biochem/physiol Actions
Livin or baculoviral IAP repeat-containing 7 (BIRC7) inhibitory functionality is mediated by the baculovirus inhibitor of apoptosis proteins (IAP)repeat (BIR) domain. This domain is also essential for interaction with caspases. The really interesting new gene (RING) finger domain elicits E3-ubiquitin ligase activity in the absence of BIR domain. The two isoforms of livin have different antiapoptotic properties, with isoform protecting cells from apoptosis induced by staurosporine and isoform α protecting cells from apoptosis induced by etoposide. An elevated expression of Livin is detected in prostate, breast, colon, head and neck squamous cell carcinoma. Inhibition of livin in nasopharyngeal carcinoma (NPC) leads to improved irradiation sensitivity in NPC cells.
Physical form
Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.
Storage and Stability
For continuous use, store at 2–8 °C for up to one month. For extended storage freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit
Quality Level	200
antibody form	affinity isolated antibody
antibody product type	primary antibodies
clone	polyclonal
form	buffered aqueous solution
mol wt	antigen ~39 kDa
species reactivity	human
packaging	antibody small pack of 25 µL
concentration	~1.0 mg/mL
application(s)	immunoprecipitation (IP): 5-10 µg using lysate of HEK-293T cells expressing human Livin","western blot: 2.5-5.0 µg/mL using whole extract of HEK-293T cells expressing human Livin
conjugate	unconjugated
UniProt accession no.	Q96CA5,
shipped in	dry ice
storage temp.	20°C
Gene Information	human ... BIRC7(79444)

WGK Germany

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