Anti-LCoR (Splice Variant)

eCl@ss:

32160702

Кат. номер

ABE1367-25UG

ABE1367-100UG

Ligand-dependent corepressor (UniProt: Q96JN0; also known as LCoR, Mblk1-related protein 2) is encoded by the LCOR (also known as C10orf12, KIAA1795, MLR2) gene (Gene ID: 84458) in human. LCoR is a ubiquitously distributed protein in fetal and adult tissues and serves as a transcriptional co-repressor that is recruited to agonist-bound nuclear receptors through a single LxxLL motif at the N-terminal region. It may also act as a transcription activator that binds DNA elements with the sequence 5-CCCTATCGATCGATCTCTACCT-3. Three isoforms of LCoR have been described that are produced by alternative splicing. This polyclonal antibody is raised against a large isoform (isoform 3; a splice variant), which associates with the PRC2 complex through its C-terminal region. Its nuclear localization signal is reported to be in amino acids 339-345 and it reacts with nuclear receptors via amino acids 53-57. (Ref.: Conway, E., et al. (2018). Mol. Cell 70(3); 408-421).
Specificity
This rabbit polyclonal antibody detects a splice variant of human and murine Ligand-dependent corepressor (LCoR). It targets an epitope with in the N-terminal half.
KLH-conjugated linear peptide corresponding to 20 amino acids from the N-terminal half of human Ligand-dependent corepressor (LCoR), isoform 3.
Epitope: unknown
Anti-LCoR, Cat. No. ABE1367, is a highly specific rabbit polyclonal antibody that targets a splice variant form of Ligand-dependent corepressor (LCoR) and has been tested in Immunocytochemistry and Western Blotting.
Research Category
Epigenetics & Nuclear Function
Western Blotting Analysis: A 1:500 dilution from a representative lot detected LCoR in whole cell lysates from Pali1 KO and matched wild type mouse embryonic stem (Courtesy of Dr. Eric Conway, Dr. Emilia Jerman & Prof. Adrian P. Bracken at the Smurfit Institute of Genetics, Trinity College Dublin, Ireland).
Western Blotting Analysis: A representative lot detected LCoR in Western Blotting applications (Conway, E., et. al. (2018). Mol. Cell. 70(3):408-421).
Quality
Evaluated by Immunocytochemistry in NTERA2 cells.
Immunocytochemistry Analysis: A 1:50 dilution of this antibody detected LCoR in NTERA2 cells.
Target description
~250 kDa observed; 170.48 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physical form
Affinity Purified
Format: Purified
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit
antibody form	affinity isolated antibody
antibody product type	primary antibodies
clone	polyclonal
species reactivity	mouse, human
packaging	antibody small pack of 25 µg
application(s)	immunocytochemistry: suitable","western blot: suitable
isotype	IgG
NCBI accession no.	NP_001333445,
UniProt accession no.	Q8N655,","Q96JN0,","Q96JN0-3,
Gene Information	human ... LCOR(84458)