Anti-LC3A/B Antibody, clone 4F11.1

eCl@ss:

32160702

Кат. номер

MABC1193-25UG

MABC1193

Microtubule-associated proteins 1A/1B light chain 3B (UniProt: Q9GZQ8; also known as Autophagy-related protein LC3 B, Autophagy-related ubiquitin-like modifier LC3 B, MAP1 light chain 3-like protein 2, MAP1A/MAP1B light chain 3 B, MAP1A/MAP1B LC3 B, Microtubule-associated protein 1 light chain 3 beta) is encoded by the MAP1LC3B (also known as MAP1ALC3) gene (Gene ID: 81631) in human. Autophagy-related protein LC3 A/B is an ubiquitin-like modifier that is involved in formation of autophagosomal vacuoles (autophagosomes). It plays a role in mitophagy that contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess reactive oxygen species production. It contains a microtubule-associated proteins 1A/1B light chain 3B (aa 2-120) and a propeptide region (aa 121-125) that is cleaved off in mature form. The precursor molecule is cleaved by ATG4B (between aa 120-121) to form the cytosolic form LC3-I, which is activated by ATG7 and then transferred to ATG3 and conjugated to phosphatidylethanolamine (PE) to generate the membrane-bound LC3-II. Phosphorylation at threonine12 by PKA inhibits conjugation to phosphatidylethanolamine (PE). Autophagy-related protein LC3 B is found to be most abundant in heart, brain, skeletal muscle and testis.
Specificity
Clone 4F11.1 detects Autophagy-related protein LC3 B in human cells.
GST/His-tagged recombinant fragment corresponding to amino acids 2-74 of human Autophagy-related protein LC3 B.
Anti-LC3A/B, clone 4F11.1, Cat. No. MABC1193, is a mouse monoclonal antibody that detects LC3A/B and has been tested for use in Western Blotting.
Research Category
Apoptosis & Cancer
Quality
Evaluated by Western Blotting in HeLa cells treated with 50 µM chloroquine.
Western Blotting Analysis: 1 µg/mL of this antibody detected LC3A/B in 10 µg of lysate from HeLa cells treated with 50 µM chloroquine.
Target description
~15 kDa observed; 14.69 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physical form
Format: Purified
Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Protein G purified
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality Level	100
biological source	mouse
antibody form	purified antibody
antibody product type	primary antibodies
clone	4F11.1, monoclonal
species reactivity	human
packaging	antibody small pack of 25 µg
application(s)	western blot: suitable
isotype	IgG2a
NCBI accession no.	NP_073729,
UniProt accession no.	Q9GZQ8,
shipped in	ambient

WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable