Anti-LbCpf1 Antibody, clone 2D5-6G11

eCl@ss:

32160702

Кат. номер

MABE1824-100UG

MABE1824-25UG

CRISPR-associated endonuclease Cas12a (UniProt: A0A182DWE3; also known as CRISPR-associated endonuclease Cpf1; Lbcpf1) is a single crRNA-guided endonuclease of the class 2 CRISPR-Cas system that lacks tracrRNA and undergoes large conformational changes upon crRNA binding. Cpf1 has several features that can distinguish it from Cas9. Unlike Cas9 that uses dual-guide RNAs, Cpf1 is guided by a crRNA. It recognizes a T-rich PAM (T-rich protospacer adjacent motif), whereas Cas9 favors a G-rich PAM and Cpf1 generates staggered ends at its PAM-distal region, but Cas9 creates blunt ends at its PAM-proximal region. Cpf1 displays higher targeting specificity in mammalian cells when compared to Cas9. It has been shown that depending on the PAM sequences, LbCpf1 can undergo conformational changes to form altered interactions with the PAM-containing DNA duplexes, hence achieving the relaxed PAM recognition. (Ref.: Zetsche, B., et al. (2015). Cell 163(3); 759-77; Yamano, T., et al. (2017). Mol. Cell 67(4); 633-645; Gao, P., et al. (2016). Cell Res. 26(8); 901-913).
Specificity
Clone 2D5-6G11 specifically detects CRISPR-associated endonuclease Cas12a (LbCpf1).
His-tagged recombinant fragment from the N-terminal region of Lachnospiracea bacteria.
Epitope: N-terminus
Western Blotting Analysis: Various dilutions from a representative lot detected LbCpf1 in lysates from HEK293 cells expressing HA-tagged LbCpf1 (Courtesy of Stefan Schuchner, Ph.D. and Egon Ogris, M.D., Medical University of Vienna, Austria).
Anti-LbCpf1, clone 2D5-6G11 Antibody, Cat. No. MABE1824, is a highly specific mouse monoclonal antibody that targets LbCpf1 and has been tested for use in Western Blotting.
Research Category
Epigenetics & Nuclear Function
Quality
Evaluated by Western Blotting in HEK293 cells expressing HA-tagged LbCpf1.
Western Blotting Analysis: 1 µg/mL of this antibody detected LbCpf1 in lysate from HEK293 cells expressing HA-tagged LbCpf1.
Target description
~144 kDa observed; 143.03 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physical form
Format: Purified
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Protein G purified
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	mouse
antibody form	purified immunoglobulin
antibody product type	primary antibodies
clone	2D5-6G11, monoclonal
species reactivity	Lachnospiraceae, bacteria
packaging	antibody small pack of 25 µg
application(s)	western blot: suitable
isotype	IgG1

WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable