Anti-LAMP1 antibody produced in rabbit

MDL number:

MFCD01864611

Кат. номер

L1418-200UL

L1418-25UL

The gene LAMP1 (lysosomal-associated membrane protein 1) encodes a type I transmembrane protein that has a short cytoplasmic tail containing a lysosome-targeting signal of GYQTI(382)-COOH. The gene is mapped to human chromosome 13q34.
Lysosome-associated membrane protein 1 (LAMP1), also termed Igp120, is a heavily glycosylated lysosomal membrane protein of about 120 kDa. It consists of a ~40 kDa core polypeptide with O-linked and 18 asparagine-linked oligosaccharide side chains. LAMP1 protein contains a leader sequence, a large intralumenal region consisting of 2 homologous domains separated by a hinge region rich in proline and serine, a 24-amino acid transmembrane region, and a short cytoplasmic tail containing the lysosomal membrane targeting signal. LAMP1 is ubiquitously expressed and highly conserved. It localizes mainly to lysosomes although a small portion is detected on the cell surface. It was found that highly metastatic tumor cells express more LAMP molecules on the cell surface than poorly metastatic cells.
synthetic peptide corresponding to amino acid residues 405-416 of human LAMP1 with N-terminal added cysteine-glycine, conjugated to KLH. The corresponding sequence is identical in rat and mouse.
Anti-LAMP1-Cy3® antibody produced in rabbit has been used in western blotting and immunofluorescence.
Biochem/physiol Actions
The gene LAMP1 (lysosomal associated membrane protein 1) encodes a membrane glycoprotein that functions as an intracellular receptor. It is found to be expressed in the cytoplasm of several types of tumor cells and may be involved in tumor invasion. Lamp1 is crucial for perforin trafficking to the lytic granules and motility of these lytic granules. Its knockdown leads to inhibition of cytotoxicity of human natural killer cells.
Lysosome-associated membrane protein 1 (LAMP1) with heavy glycosylation, may be important to protect the lysosomal membrane from proteolytic enzymes within lysosomes.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
CAB-O-SIL is a registered trademark of Cabot Corp.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit
Quality Level	200
antibody form	affinity isolated antibody
antibody product type	primary antibodies
clone	polyclonal
form	buffered aqueous solution
mol wt	antigen ~120 kDa
species reactivity	mouse, human, rat
packaging	antibody small pack of 25 µL
application(s)	indirect immunofluorescence: 5-10 µg/mL using human HeLa, rat NRK, and mouse NIH3T3 cells
conjugate	unconjugated
UniProt accession no.	P11279,
shipped in	dry ice
storage temp.	20°C
Gene Information	human ... LAMP1(3916) mouse ... Lamp1(16783) rat ... Lamp1(25328)

Personal Protective Equipment	Eyeshields,, Gloves,, multi-purpose combination respirator cartridge (US),
RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable