Anti-LAMP-1 (CD107a) Antibody

eCl@ss:	32160702
NACRES:	NA.41

Кат. номер

AB2971-25UG

AB2971

Lysosome-associated membrane protein 1 (LAMP-1) is a highly glycosolated, single-pass type 1 membrane protein belonging to the lysosome- associated membrane protein family; a group of proteins that make up the majority of the lysosomal membrane and are considered crucial mediators for cellular adhesion by interacting with carbohydrate receptors. LAMP-1 functions to traffic carbohydrate ligands to selectins. It is also thought to play a role in tumor cell metastasis.
Specificity
The antibody recognizes LAMP-1 at the topological domain (Lumenal).
KLH-conjugated linear peptide corresponding to human LAMP-1 at the topological domain (Lumenal).
Epitope: Topological domain (Lumenal)
Research Sub Category
Apoptosis - Additional
Tumor Markers
This Anti-LAMP-1 (CD107a) Antibody is validated for use in WB, IC for the detection of LAMP-1 (CD107a).
Research Category
Apoptosis & Cancer
Western Blot (Snap i.d.) Analysis: 1 µg/mL from a previous lot detected LAMP-1 in 10 µg of EL4 cell lysate.
Immunocytochemistry Analysis: 1:500 dilution from a previous lot detected LAMP-1 in NIH/3T3, A431, and HeLa cells.
Quality
Evaluated by Western Blot in EL4 cell lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected LAMP-1 in 10 µg of EL4 cell lysate.
Target description
~ 95kDa observed. Although the calculated MW 45 kDa, LAMP-1 is heavily glycosylated and therefore, MW typically can be expected between 90-120 kDa Uncharacterized bands may be observed in some lysates.
Physical form
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Affinity purified
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
EL4 cell lysate
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit
Quality Level	100
antibody form	affinity isolated antibody
antibody product type	primary antibodies
clone	polyclonal
purified by	affinity chromatography
species reactivity	mouse, rat, human
species reactivity (predicted by homology)	rhesus macaque (based on 100% sequence homology)
packaging	antibody small pack of 25 µg
application(s)	immunocytochemistry: suitable","western blot: suitable
NCBI accession no.	NP_005552,
UniProt accession no.	P11279,
shipped in	ambient
storage temp.	2-8°C

WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable