Anti-Lamin A/C Antibody, clone 4C11

eCl@ss:

32160702

Кат. номер

MABT1341-25UG

MABT1341-100UG

Prelamin-A/C (UniProt: P02545) is encoded by the LMNA (also known as LMN1) gene (Gene ID: 4000) in human. It is cleaved into Lamin-A/C (also known as 70 kDa Lamin, Renal carcinoma antigen NY-REN-32). Lamins are components of the nuclear lamina that provide a framework for the nuclear envelope and may also interact with chromatin. Lamin A and C are present in equal amounts in the lamina of mammals. Plays an important role in nuclear assembly, chromatin organization, nuclear membrane and telomere dynamics. Lamin A is initially synthesized as prelamin A that undergoes several modifications in the carboxyl terminal region that allow incorporation of prelamin A into the nuclear envelope and its subsequent processing into the mature lamin A. Cleavage of 15 residues (aa 647-662) by ZMPSTE24/FACE1 generates the final protein product. Unlike mature lamin A, prelamin A accumulates as discrete and localized foci at the nuclear periphery. Prelamin-A/C can accelerate smooth muscle cell senescence. It can act to disrupt mitosis and induce DNA damage in vascular smooth muscle cells (VSMCs), leading to mitotic failure, genomic instability, and premature senescence. Mutations in LMNA gene are known to cause Emery-Dreifuss muscular dystrophy that is characterized by weakness and atrophy of muscle without involvement of the nervous system. Some mutations have also been linked to familial type of lipodystrophy characterized by the loss of subcutaneous adipose tissue in the lower parts of the body. (Ref.: Casasola, A., et al. (2016). Nucleus 7(1); 84-102).
Specificity
Clone 4C11 detects Lamin A/C at the nuclear lamina in multiple species.
Purified protein corresponding to the Ig-fold of human Lamin A/C.
Research Category
Cell Structure
Western Blotting Analysis: A representative lot detected Lamin A/C in Western Blotting applications (Gesson, K., et. al. (2016). Genome Res. 26(4):462-73; Roblek, M., et. al. (2010). PLoS One. 5(5):e10604).
Dot Blot Analysis: A representative lot detected Lamin A/C in Dot Blot applications (Roblek, M., et. al. (2010). PLoS One. 5(5):e10604).
Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected Lamin A/C in HeLa cells.
Western Blotting Analysis: A representative lot detected Lamin A/C in WB used to demonstrate high titration and excellent properties of clone 4C11 (Courtesy of Marie lang, M.D., Stefan Schuchner, Ph.D. and Egon Ogris, M.D., Medical University of Vienna, Austria).
Immunofluorescence Analysis: A representative lot detected the Lamin A/C species at the nuclear lamina of HeLa cells (Courtesy of Marie Lang, M.D., Stefan Schuchner, Ph.D. and Egon Ogris, M.D., Medical University of Vienna, Austria).
Immunoprecipitation Analysis: A representative lot detected Lamin A/C in WB used to demonstrate high titration and excellent properties of clone 4C11 (Courtesy of Marie Lang, M.D., Stefan Schuchner, Ph.D. and Egon Ogris, M.D., Medical University of Vienna, Austria).
Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected Lamin A/C in Chromatin Immunoprecipitation applications (Gesson, K., et. al. (2016). Genome Res. 26(4):462-73).
Immunofluorescence Analysis: A representative lot detected Lamin A/C in Immunofluorescence applications (Roblek, M., et. al. (2010). PLoS One. 5(5):e10604).
Immunoprecipitation Analysis: A representative lot immunoprecipitated Lamin A/C in Immunoprecipitation applications (Gesson, K., et. al. (2016). Genome Res. 26(4):462-73).
Anti-Lamin A/C, clone 4C11, Cat. No. MABT1341, is a highly specific mouse monoclonal antibody that targets Lamin A/C and has been tested for use in Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP), and Western Blotting.
Quality
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: 0.2 µg/mL of this antibody detected Lamin A/C in HeLa cell lysate.
Target description
74 and 65 kDa observed. 74.14 kDa and 65.14 kDa calculated for Lamin A and C, respectively. Uncharacterized bands may be observed in some lysate(s).
Physical form
Format: Purified
Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Protein G purified
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	mouse
antibody form	purified immunoglobulin
antibody product type	primary antibodies
clone	4C11, monoclonal
species reactivity	human, Syrian hamster, rat, mouse, monkey
packaging	antibody small pack of 25 µg
application(s)	ChIP: suitable","immunofluorescence: suitable","immunoprecipitation (IP): suitable","western blot: suitable
isotype	IgG2a
NCBI accession no.	NP_001244303,
UniProt accession no.	P02545,
Gene Information	human ... LMNA(4000)

WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable