Anti-Ketohexokinase Isoform C Antibody, clone 5F1-3F5

eCl@ss:

32160702

Кат. номер

MABS1907-25UG

MABS1907-100UG

Ketohexokinase (UniProt: P50053; also known as EC: 2.7.1.3, Hepatic fructokinase) is encoded by the KHK gene (Gene ID: 3795) in human. Ketohexokinase is a homodimeric cytosolic enzyme in that catalyzes the conversion of fructose to fructose 1 phosphate. It is most abundant in the liver, where it may constitute up to 0.6% of the total liver protein. It has also been reported in kidney, spleen, gut, and pancreas. Very low levels are also found in adrenal, muscle, brain, and eyes. It has multiple fructose binding sites. There are two major isoforms of this enzyme ketofructokinase C and ketofructokinase A. Due to its high Km (~ 28 mM), fructose is preferentially metabolized by the C isoform. Ketohexokinase -C is unique among sugar kinases in that its metabolism of fructose is associated with a rapid depletion of intracellular ATP. Unlike glucokinase, in which excessive phosphorylation of glucose is prevented by a negative feedback system, the metabolism of fructose by ketohexokinase-C results in rapid phosphorylation with a fall in intracellular phosphate and ATP. If the ATP levels fall significantly then the protein synthesis is transiently stopped and cells may develop features that are consistent with ischemia. Mutations in KHK gene has been linked to fructosuria, a clinically benign condition that is characterized nu the incomplete metabolism of fructose in the liver, leading to its urinary excretion. (Ref.: Diggle, CP et al., (2009). J. Histochem. Cytochem. 57(8): 763-774; Hayward, BE., and Bonthron, DT (1998). Eur. J. Biochem. 257(1); 85-91).
Specificity
Clone 5F1-3F5 specifically detects isoform C of human ketohexokinase.
A full-length human recombinant Ketohexokinase, isoform C.
Western Blotting Analysis: 2 µg/mL from a represenative lot detected Ketohexokinase Isoform C in HEK293 cells expressing HA-tagged KHK-C.
Western Blotting Analysis: 1 µg/mL from a represenative lot detected Ketohexokinase Isoform C in human liver tissue lysate.
Research Category
Signaling
Anti-Ketohexokinase Isoform C, clone 5F1-3F5, Cat. No. MABS1907, is a mouse monoclonal antibody that detectsKetohexokinase and has been tested for use in Western Blotting.
Quality
Evaluated by Western Blotting in human kidney tissue lysate.
Western Blotting Analysis: 2 µg/mL of this antibody detected Ketohexokinase Isoform C in human kidney tissue lysate.
Target description
~33 kDa observed; 32.52 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physical form
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G purified
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	mouse
antibody form	purified immunoglobulin
antibody product type	primary antibodies
clone	5F1-3F5, monoclonal
species reactivity	human
packaging	antibody small pack of 25 µg
application(s)	western blot: suitable
isotype	IgG1
NCBI accession no.	NP_000212,
UniProt accession no.	P50053-1,