Anti-IQGAP1 (C-terminal region) antibody produced in rabbit

Кат. номер

SAB4200079-25UL

SAB4200079-200UL

IQ motif containing GTPase activating protein 1(IQGAP1) is encoded by the gene mapped to human chromosome 15q26.1. The encoded 190kDa protein is widely expressed and is mainly localized to lamellipodia and membrane ruffles in fibroblasts and other cell types.
IQGAP1 is a scaffolding protein and is made up of N-terminal calponin-homology domain (CHD), WW domain and guanosine triphosphatase (GTPase) activating protein GAP-related domain (GRD).
Specificity
Anti-IQGAP1 (C-terminal region) specifically recognizes human IQGAP1.
Anti-IQGAP1 (C-terminal region) antibody produced in rabbit may be used in:

• immunoprecipitation
• western blotting
• immunohistochemistry

Biochem/physiol Actions
IQ motif containing GTPase activating protein 1(IQGAP1) interacts with cell division control protein 42 homolog (Cdc42) or Rac1 and regulate cadherin-based cell adhesion. IQGAP1 binds to NLRC3 in human monocytic and epithelial cells and regulate type I interferon (IFN) production. Overexpression of the gene has been observed in gastric cancer cell lines.In addition, IQGAP1 is also associated with diseases, such as cancer and diabetes. The encoded protein is also implicated in the production and secretion of insulin. IQGAP1 also aids in the endocytosis and plasma membrane fusion of vesicles in pancreatic β-cells.
IQGAP1 plays a role in tumorigenesis. IQGAP1 gene is upregulated in several carcinomas, including colorectal, lung, ovary, and breast carcinoma.
Physical form
Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.
Storage and Stability
Store at –20 °C. For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit
Quality Level	200
antibody form	affinity isolated antibody
antibody product type	primary antibodies
clone	polyclonal
form	buffered aqueous solution
mol wt	antigen ~189 kDa
species reactivity	human
packaging	antibody small pack of 25 µL
concentration	~1.5 mg/mL
application(s)	immunohistochemistry: 20-40 µg/mL using formalin-fixed and paraffin embedded human colon","immunoprecipitation (IP): 10-20 µg using HeLa cell lysates","western blot: 2-4 µg/mL using using MCF7 cell extracts
conjugate	unconjugated
UniProt accession no.	P46940,
shipped in	dry ice
storage temp.	20°C
Gene Information	human ... IQGAP1(8826)

RIDADR	NONH for all modes of transport
Flash Point F	Not applicable
Flash Point C	Not applicable