Anti-Inntag 5 (IT5) Antibody, clone R19/8-11/18

eCl@ss:	32160702
NACRES:	NA.41

Кат. номер

MAC138-25UL

MAC138

Pollen allergen Phl p 2 (UniProt: P43214; also known as Allergen Phl p II, Phl p 2) is encoded by the PHLPII gene in Phleum pratense (Common timothy). Phl p2 binds to IgE and causes allergic reactions in human. Inntag 5 IT5 is a small protein domain of Phelum pratense that display strong structural determinants and does not generate solubility issues in vivo and in vitro. Also, it is not shown to compromise cell fitness when overexpressed and has no detectable functional and localization effects when fused to a variety of target proteins. Although the Inntag IT5 has a similar length to that of the Flag tag, it does not affect cell morphology and produces only very mild effect in the mean critical size at the G1/S transition compared to Flag tag. The structure of Inntag IT5 is based on a packed beta-sheet confirmation.
Specificity
Clone R19/8-11/18 detects Pollen allergen Phl p 2 in lysate from HEK293 cells transfected with GFP-IT5.
His-tagged recombinant fragment corresponding to 96 amino acids of Pollen allergen Phl p 2 chain.
Research Category
Secondary & Control Antibodies
Western Blotting Analysis: A representative lot detected Inntag 5 (IT5) in Western Blotting applications (Georgieva, M.V., et. al. (2015). Nat Methods. 12(10):955-8).
Immunoprecipitation Analysis: A representative lot detected Inntag 5 (IT5) in Immunoprecipitation applications (Georgieva, M.V., et. al. (2015). Nat Methods. 12(10):955-8).
Immunofluorescence Analysis: A representative lot detected Inntag 5 (IT5) in Immunofluorescence applications (Georgieva, M.V., et. al. (2015). Nat Methods. 12(10):955-8).
Anti-Inntag 5 (IT5), clone R19/8-11/18 is a mouse monoclonal antibody that detects the Innocuous Inntag IT5 based on pollen allergen PhIp2 sequence and is tested for Immunofluorescence, Immunoprecipitation, and Western Blotting.
Quality
Evaluated by Western Blotting in transfected HEK293/GFP-IT5 cell lysates.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Inntag 5 (IT5) in 10 µg of transfected HEK293/GFP-IT5 cell lysates.
Target description
~40.2 kDa observed; 13.36 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physical form
Purified mouse monoclonal antibody IgG2b in PBS without azide.
Format: Purified
Protein G purified
Storage and Stability
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality Level	100
biological source	mouse
antibody form	purified immunoglobulin
antibody product type	primary antibodies
clone	R19/8-11/18, monoclonal
species reactivity	Phleum pratense
species reactivity (predicted by homology)	all
packaging	antibody small pack of 25 µL
application(s)	immunofluorescence: suitable","immunoprecipitation (IP): suitable","western blot: suitable
isotype	IgG2b
UniProt accession no.	P43214,
shipped in	ambient

WGK Germany	WGK 2
Flash Point F	Not applicable
Flash Point C	Not applicable