Anti-HYAL1 Antibody, clone 1D10

Кат. номер

MABT1438-25UL

MABT1438-100UL

Hyaluronidase-1 (UniProt: Q12794; also known as EC:3.2.1.35, Hyal-1, Hyaluronoglucosaminidase-1, Lung carcinoma protein 1, LuCa-1) is encoded by the HYAL1 (also known as LUCA1) gene (Gene ID: 3373) in human. Hyaluronidases (HYAL) catalyze the breakdown of hyaluronic acid at the β1-4 linkage between N-acetyl-β-D-glucosamine and D-glucuronic acid to generate smaller oligosaccharides. HYAL is an acid hydrolase with a pH optimum of ~ 3.8. It is synthesized with a signal peptide (aa 1-21), which is subsequently cleaved off to generate the mature form that is mainly present in the lysosomal compartment. In murine macrophages, HYAL1 is reportedly trafficked to the lysosomes by a Man-6-P-independent secretion/recapture mechanism mediated by the mannose receptor. Its expression is higher in the liver, kidney, and heart and weak expression is observed in lung, placenta, and skeletal muscle. It has also been detected in plasma and in urine. HYAL-1 is also responsible for the production of angiogenic HA fragments. Its presence in urine and change in expression level in bladder and prostate makes in a marker of bladder and prostate cancers. Significantly higher expression of HYA1 is observed in advanced stages of bladder cancer. Inactivating mutations of HYAL1 gene have been linked to mucopolysaccharidosis IX that is characterized by diminished stature and periarticular soft tissue masses with 40 to 90-fold higher levels of hyaluronic acid than in normal serum. (Ref.: Puissant, E., et al. (2014). Traffic. 15(5); 500-515; Chao, KL., et al. (2007). Biochemistry. 46(23); 6911-6920).
Specificity
Clone 1D10 is a mouse monoclonal antibody that detects Hyaluronidase-1.
His-tagged recombinant fragment corresponding to the first 366 amino acids from human Hyaluronidase-1.
Quality Control Testing
Evaluated by Western Blotting in Mouse kidney tissue lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected Hyaluronidase-1 in Mouse kidney tissue lysate.
Tested Applications
Western Blotting Analysis: A 1:500 dilution from a representative lot detected HYAL1 in Mouse liver tissue lysate.
Western Blot using KO lysates: A 1:500 dilution from a representative lot detected HYAL1 in lysate from wild-type liver tissue, but not in mouse liver with HYAL1 knockout. (Data courtesy of Dr. Barbara Triggs-Raine, Ph.D., University of Manitoba, Canada).
Western Blotting Analysis: A representative lot detected HYAL1 in Western Blotting application. (Shuttleworth, T., et al. (2002). J Biol Chem. 277(25):23008-18; Martin, D.C., et al. (2008). Hum Mol Genet. 17(13):1904-15; Hemming, R., et al. (2008). Glycobiology. 18(4):280-9; Puissant, E., et al. (2014). Traffic. 15(5):500-15).
Immunohistochemistry Applications: A representative lot detected HYAL1 in Immunohistochemistry application. (de Sa, V.K., et al. (2015). Braz J Med Biol Res. 48(11):1039-47).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-HYAL1, clone 1D10, Cat. No. MABT1438, is a mouse monoclonal antibody that detects Hyaluronidase-1 and is tested for use in Immunohistochemistry, Western Blotting, and Western Blotting using knockout lysate.
Physical form
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality Level	200
biological source	mouse
antibody form	purified antibody
antibody product type	primary antibodies
clone	1D10, monoclonal
mol wt	calculated mol wt 48.37 kDa","observed mol wt ~52 kDa
purified by	using protein G
species reactivity	hamster, human, mouse
packaging	antibody small pack of 100 µL
application(s)	immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable","western blot: suitable
isotype	IgG1
epitope sequence	Unknown
conjugate	unconjugated
Protein ID accession no.	NP_149349,
UniProt accession no.	Q12794,
shipped in	2-8°C
Gene Information	human ... HYAL1(3373)

WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable