Anti-Human CD40 (G28.5) ColorWheel® Dye-Ready mAb CWA-1023-25UL

Specificity
Clone G28.5 is a mouse monoclonal antibody that detects human Tumor necrosis factor receptor superfamily member 5 (CD40). It targets an epitope with in D1/B2 domain in the extracellular region.
Human erythrocyte-rosette-negative tonsillar lymphocytes.
Quality Control Testing:
Evaluated by Flow Cytometry in Human peripheral blood mononuclear cells (PBMC).
Flow Cytometry Analysis: Staining of one million Human peripheral blood mononuclear cells (PBMC) gated for lymphocytes was performed using 5 µL of a 1:1 mixture of Cat. No. CWA-1023, Anti-CD40 Antibody (G28.5) Colorwheel® Mouse mAb and Cat. No. CWD-PE Colorwheel® activated Phycoerythrin (PE) Dye or an equivalent amount of PE-conjugated Mouse IgG1 isotype control.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-Human CD40 (G28.5) ColorWheel<® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any Colorwheel® antibody with any Colorwheel® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each Colorwheel® antibody and dye is lyophilized for long long-term storage, allowing for simplicity and flexibility without compromise
Target description
Tumor necrosis factor receptor superfamily member 5 (UniProt: P25942; also known as B-cell surface antigen CD40, Bp50, CD40L receptor, CDw40, CD40) is encoded by the CD40 (also known as TNFRSF5) gene (Gene ID: 958) in human. CD40, a member of the TNFR super family, is a single-pass type I membrane protein that is synthesized with a signal peptide (aa 1-20 ), which is subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 21-193), a transmembrane domain (aa 194-215), and a cytoplasmic domain (aa 216-277). It has multiple cysteine-rich repeats. CD40 is expressed in B-cells, monocytes, dendritic cells, thymic epithelium as well as on certain carcinomas. It serves as a receptor for CD40L that is transiently expressed on T-cells and some non-immune cells under inflammatory conditions. Upon engagement with its ligand, CD40 directly or indirectly recruits TRAF1, TRAF2, TRAF3, TRAF5, and TRAF6 to its cytoplasmic domains. The canonical consensus binding sites for TRAF1, TRAF2, and TRAF3 are situated at the membrane distal domain of the intracellular tail of CD40 and is defined by the amino acid sequence PxQxT. CD40 transduces TRAF6 and MAP3K8-mediated signals that activate ERK in macrophages and B cells, leading to induction of immunoglobulin secretion. Clone G28.5 is reported to bind to an epitope localized in D1/B2 domains in the extracellular cysteine rich region and is a potent activator of B-cells. Mutations in CD40 gene are known to cause immunodeficiency with hyper-IgM3 (HIGM3) that is characterized by normal or elevated serum IgM levels with absence of IgG, IgA, and IgE. This results in a profound susceptibility to bacterial infections. (Ref.: Elgueta, R., et al. (2009). Immunol. Rev. 229(1); 152-172; Malmborg Hager, AC., et al. (2003). Scand. J, Immunol. 57(6); 517-524).
Physical form
Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted antibody solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.
Reconstitution
1.0 mg/mL after reconstitution at 25 μL in PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL of PBS. Protect from light.
ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany

Quality Level	200
biological source	mouse
antibody form	purified antibody
antibody product type	primary antibodies
clone	G28.5, monoclonal
product line	ColorWheel®
form	lyophilized
mol wt	calculated mol wt 30.62 kDa
species reactivity	human
packaging	antibody small pack of 25 µL
application(s)	flow cytometry: suitable
isotype	IgG1
epitope sequence	Extracellular domain
Protein ID accession no.	AAH64518.1,
UniProt accession no.	P25942,
shipped in	ambient
storage temp.	2-8°C
Gene Information	human ... CD40(958)