Anti-Human CD36 (CB38) ColorWheel® Dye-Ready mAb CWA-1025-25UL

Specificity
Clone CB38 is a mouse monoclonal antibody that detects human platelet glycoprotein 4 (CD36).
Human myeloid cells.
Quality Control Testing:
Evaluated by Flow Cytometry in Human peripheral blood mononuclear cells (PBMC).
Flow Cytometry Analysis: Staining of one million Human peripheral blood mononuclear cells (PBMC) was performed using 5 µL of a 1:1 mixture of Cat. No. CWA-1025, Anti-CD36 Antibody (CB38) Colorwheel® Mouse mAb and Cat. No. CWD-PE Colorwheel® activated Phycoerythrin (PE) Dye or an equivalent amount of PE-conjugated Mouse IgM isotype control.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-Human CD36 (CB38) ColorWheel<® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any Colorwheel® antibody with any Colorwheel® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each Colorwheel® antibody and dye is lyophilized for long long-term storage, allowing for simplicity and flexibility without compromise
Target description
Platelet glycoprotein 4 (UniProt: P16671; also known as Fatty acid translocase, FAT, Glycoprotein IIIb, GPIIIB, Leukocyte differentiation antigen CD36, PAS IV, PAS-4, Platelet collagen receptor, Platelet glycoprotein IV, GPIV, Thrombospondin receptor, CD36) is encoded by the CD36 (also known as GP3B, GP4) gene (Gene ID: 948) in human. CD36 is a multi-pass membrane glycoprotein that contains two cytoplasmic domains (aa 2-7 and 462-472), one extracellular domain (aa 30-439), and two transmembrane domains (aa 8-29 and 440-461). It acts as a receptor for a broad range of ligands that can be of proteinaceous nature like thrombospondin, fibronectin, collagen, and amyloid-beta or of lipidic nature, such as oxidized low-density lipoprotein (oxLDL), anionic phospholipids, long-chain fatty acids and bacterial diacylated lipopeptides. They are generally multivalent and can engage multiple receptors simultaneously. The resulting formation of CD36 clusters initiates signal transduction and internalization of receptor-ligand complexes. Cellular responses to these ligands are involved in angiogenesis, inflammatory response, fatty acid metabolism, and dietary fat processing in the intestine. It binds long-chain fatty acids and facilitates their transport into cells. In taste receptor cells, CD36 is reported to mediate an increase in intracellular calcium levels by long-chain fatty acids, leading to the activation of the gustatory neurons. Upon binding of oxLDL or amyloid peptide 42, it interacts with the TLR4:TLR6 heterodimer and the complex is then internalized and triggers inflammatory response, leading to NF-kB-dependent production of several cytokines. Mutations in CD36 gene are associated with Platelet glycoprotein IV deficiency that is characterized by macrothrombocytopenia.
Physical form
Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted antibody solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.
Reconstitution
1.0 mg/mL after reconstitution at 25 μL in PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL of PBS. Protect from light.
ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany

Quality Level	200
biological source	mouse
antibody form	purified antibody
antibody product type	primary antibodies
clone	CB38, monoclonal
product line	ColorWheel®
form	lyophilized
mol wt	calculated mol wt 53.05 kDa
species reactivity	human
packaging	antibody small pack of 25 µL
application(s)	flow cytometry: suitable
isotype	IgM
epitope sequence	Extracellular domain
Protein ID accession no.	NP_002380.3,
UniProt accession no.	P16671,
shipped in	ambient
storage temp.	2-8°C
Gene Information	human ... CD36(948)