Anti-Human CD35 (E11) ColorWheel® Dye-Ready mAb CWA-1024-25UL

Specificity
Clone E11 is a mouse monoclonal antibody that detects human Complement receptor type 1 (CD35).
Acute monocytic leukemia cells and normal human blood monocytes.
Quality Control Testing:
Evaluated by Flow Cytometry in Human peripheral blood mononuclear cells (PBMC).
Flow Cytometry Analysis: Staining of one million Human peripheral blood mononuclear cells (PBMC) was performed using 5 µL of a 1:1 mixture of Cat. No. CWA-1024, Anti-CD35 Antibody (E11) Colorwheel® Mouse mAb and Cat. No. CWD-PE Colorwheel® activated Phycoerythrin (PE) Dye or an equivalent amount of PE-conjugated Mouse IgG1 isotype control.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-Human CD35 (E11) ColorWheel<® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any Colorwheel® antibody with any Colorwheel® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each Colorwheel® antibody and dye is lyophilized for long long-term storage, allowing for simplicity and flexibility without compromise
Target description
Complement receptor type 1 (UniProt: P17927; also known as C3b/C4b receptor, CD35) is encoded by the CR1 (also known as C3BR) gene (Gene ID: 1378) in human. CD35 is a single-pass type I membrane glycoprotein of the receptors of complement activation (RCA) family. It is synthesized with a signal peptide (aa 1-42), which is subsequently cleaved off to generate the mature form that contains a long extracellular domain (aa 42-1971) , a transmembrane domain (aa 1972-1996), and a cytoplasmic domain (aa 1997-2039). It contains 30 Sushi domains. Its expression has been reported on erythrocytes, a subset of T-cells, mature B cells, follicular dendritic cells, monocytes, and granulocytes. It serves as a membrane immune adherence receptor that plays a critical role in the capture and clearance of complement -opsonized pathogens by erythrocytes and monocytes/macrophages. Its ligands include C3b, iC3b, and C4b fragments of C3 and C4 proteins. CD35 also acts as an inhibitor of spontaneous complement activation by impairing the formation and function of the alternative and classical pathways C3/C5 convertases, and by serving as a cofactor for the cleavage of C3b to iC3b, C3c, and C3d, C3g, and C4b, C4c, and C4d by factor I. It also plays a role in immune regulation by contributing, upon ligand binding, to the generation of regulatory T cells from activated helper T cells and inn maintaining a balance between normal and pathological B-cell activation. (Ref.: Mcsik-Valent, B., et al. (2019). Front. Immunol. 10; 1493; Kremlitzka, M., et al. (2013). Int. Immunol. 25(1); 25-33; Schifferli, JA., et al. (1988). J. Immunol. 140(3); 899-904).
Physical form
Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted antibody solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.
Reconstitution
1.0 mg/mL after reconstitution at 25 μL in PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL of PBS. Protect from light.
ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany

Quality Level	200
biological source	mouse
antibody form	purified antibody
antibody product type	primary antibodies
clone	E11, monoclonal
product line	ColorWheel®
form	lyophilized
mol wt	calculated mol wt 223.66 kDa
species reactivity	human
species reactivity (predicted by homology)	nonhuman primates
packaging	antibody small pack of 25 µL
application(s)	flow cytometry: suitable
isotype	IgG1
epitope sequence	Extracellular domain
Protein ID accession no.	NP_002380.3,
UniProt accession no.	P17927,
shipped in	ambient
storage temp.	2-8°C
Gene Information	human ... CR1(1378)