Anti-Human CD29 (MEM-101A) ColorWheel® Dye-Ready mAb CWA-1062-25UL

Colorwheel® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any Colorwheel® antibody with any Colorwheel® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each Colorwheel® antibody and dye is lyophilized for long long-term storage, allowing for simplicity and flexibility without compromise
Specificity
Clone MEM-101A is a mouse monoclonal antibody that detects Integrin b1 (CD29). It targets an epitope within the extracellular domain.
Raji Burkitt′s lymphoma cells.
Quality Control Testing:
Evaluated by Flow Cytometry in Human peripheral blood mononuclear cells (PBMC).
Flow Cytometry Analysis (FC): Staining of one million Human peripheral blood mononuclear cells (PBMC) was performed using 5 µL of a 1:1 mixture of Cat. No. CWA-1062, Anti-Human CD29 (MEM-101A) Colorwheel® Dye-Ready mAb and Cat. No. CWD-PE Colorwheel® Antibody-Ready Phycoerythrin (PE) Dye or an equivalent amount of PE-conjugated Mouse IgG1 isotype control.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-Human CD29 (MEM-101A) Colorwheel® Dye-Ready mAb, Cat. No. CWA-1062, is a mouse monoclonal antibody that detects CD29 and is tested for use in Flow Cytometry.
Target description
Integrin beta-1 (UniProt: P05556; also known as Fibronectin receptor subunit beta, Glycoprotein IIa, GPIIA, VLA-4 subunit beta, CD29 is encoded by the ITGB1 (also known as FNRB, MDF2, MSK12) gene (Gene ID: 3688) in human. Integrins are heterodimeric integral membrane proteins composed of a variable a subunit and a conserved b subunit that function in cell surface adhesion and signaling. They contain a large extracellular domain responsible for ligand binding, a single transmembrane domain, and a cytoplasmic domain. The exact combination of various a- and b-subunits dictates the binding specificity of integrins to different ECM components. Although both subunits are required for adhesion, the binding specificity primarily depends on the extracellular portion of the a-subunit. Integrin b1 is a single-pass type I membrane glycoprotein that associates with one of the a subunits to form receptor for fibronectin. It recognizes the sequence R-G-D in a wide array of ligands. Five isoforms of integrin b1 have been reported that are produced by alternative splicing. Integrin b1 is synthesized with a signal peptide (aa 1-20) that is subsequently cleaved off. The mature form has an extracellular domain (aa 21-728), a helical domain (aa 729-751) and a cytoplasmic region (aa 752-798). Isoform 1 of integrin b1 is widely expressed and other isoforms are generally co-expressed with a more restricted distribution. Isoform 2 is expressed in skin, liver, skeletal muscle, cardiac muscle, placenta, umbilical vein endothelial cells, neuroblastoma cells, lymphoma cells, hepatoma cells and astrocytoma cells. Isoform 3 and isoform 4 are expressed in muscle, kidney, liver, placenta, cervical epithelium, umbilical vein endothelial cells, fibroblast cells, embryonal kidney cells, platelets and several blood cell lines. Isoform 4 is selectively expressed in peripheral T-cells and isoform 5 is expressed specifically in striated muscle (skeletal and cardiac muscle). Isoform 2 is reported to interfere with isoform 1 resulting in a dominant negative effect on cell adhesion and migration (in vitro). (Ref.: Balcaz, A., et al. (1993). J. Cell Biol. 121(1); 171-178).
Physical form
Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted antibody solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.
Reconstitution
1.0 mg/mL after reconstitution at 25 μL in PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL of PBS. Protect from light.
ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality Level	200
biological source	mouse
antibody form	purified antibody
antibody product type	primary antibodies
clone	MEM-101A, monoclonal
product line	ColorWheel®
form	lyophilized
mol wt	calculated mol wt 88.42 kDa
species reactivity	human
species reactivity (predicted by homology)	porcine, canine
packaging	antibody small pack of 25 µL
application(s)	flow cytometry: suitable
isotype	IgG1
epitope sequence	Extracellular domain
Protein ID accession no.	NP_596867.1,
UniProt accession no.	P05556,
shipped in	ambient
storage temp.	2-8°C
Gene Information	human ... ITGB1(3688)