Anti-Human CD235a (HIR-2) ColorWheel® Dye-Ready mAb CWA-1028-25UL

Specificity
Clone HIR-2 is a mouse monoclonal antibody that detects human CD235a (Glycophorin-A). It targets an epitope within the N-terminal region.
Synthetic peptide from the N-terminal region of human CD235a.
Quality Control Testing:
Evaluated by Flow Cytometry in K562 cell line.
Flow Cytometry Analysis: Staining of one million K562 cells was performed using 5 µL of a 1:1 mixture of Cat. No. CWA-1028, Anti-Human CD235a (HIR-2) Colorwheel® Dye-Ready mAb and Cat. No. CWD-PE Colorwheel® activated Phycoerythrin (PE) Dye or an equivalent amount of PE-conjugated Mouse IgG2b isotype control.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-Human CD235a (HIR-2) ColorWheel<® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any Colorwheel® antibody with any Colorwheel® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each Colorwheel® antibody and dye is lyophilized for long long-term storage, allowing for simplicity and flexibility without compromise
Target description
Glycophorin-A (UniProt: P02724; also known as MN sialoglycoprotein, PAS-2, Sialoglycoprotein alpha, CD235a) is encoded by the GYPA (also known as GPA) gene (Gene ID: 2993) in human. Glycophorins are heavily glycosylated sialoglycoproteins and four different types have been described in humans: Glycophorins: A (CD235a), B, C and D. CD235a is a homodimeric, single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-19), which is subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 20-91), a transmembrane domain (aa 92-114), and a cytoplasmic domain (aa 115-150). The extracellular domain contains one N-linked and sixteen O-linked oligosaccharide chains terminating in sialic acid residues that display negative charges on the red cell surface. CD235a is a major intrinsic membrane protein of the erythrocyte. In its N-terminal glycosylated segment, it has MN blood group receptors that are under the control of an autosomal locus found on chromosome 4, with two alleles LM and LN. CD235a appears to be important for the function of solute carrier family 4 member 1 (SLC4A1) and may be involved in translocation of SLC4A1 to the plasma membrane. It also functions as a complement regulator and a receptor for bacteria, viruses, and Plasmodium falciparum. (Ref.: Jaskiewicz, J., et al. (2019). Parasites & Vectors. 12; Article 317; Hassoun, H., et al. (1998). Blood. 91(6); 46-51).
Physical form
Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted antibody solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.
Reconstitution
1.0 mg/mL after reconstitution at 25 μL in PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL of PBS. Protect from light.
ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany

Quality Level	200
biological source	mouse
antibody form	purified antibody
antibody product type	primary antibodies
clone	HIR-2, monoclonal
product line	ColorWheel®
form	lyophilized
mol wt	calculated mol wt 16.33 kDa
species reactivity	human
packaging	antibody small pack of 25 µL
application(s)	flow cytometry: suitable
isotype	IgG2b
epitope sequence	N-terminal
Protein ID accession no.	NP_002090.4 ,
UniProt accession no.	P02724,
shipped in	ambient
storage temp.	2-8°C
Gene Information	human ... GYPA(2993)