Anti-Human CD2 (TS1/8) ColorWheel® Dye-Ready mAb CWA-1027-25UL

Specificity
Clone TS1/8 is a mouse monoclonal antibody that detects human T-cell surface antigen CD2 (CD2).
Cytotoxic T lymphocytes
Anti-Human CD2 (TS1/8) ColorWheel<® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any Colorwheel® antibody with any Colorwheel® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each Colorwheel® antibody and dye is lyophilized for long long-term storage, allowing for simplicity and flexibility without compromise
Quality Control Testing:
Evaluated by Flow Cytometry in Human PBMCs.
Flow Cytometry Analysis: Staining of one million Human peripheral blood mononuclear cells (PBMC) was performed using 5 µL of a 1:1 mixture of Cat. No. CWA-1027, Anti-Human CD2 (TS1/8) Colorwheel® Dye-Ready mAb and Cat. No. CWD-PE Colorwheel® activated Phycoerythrin (PE) Dye or the equivalent amount of Phycoerythrin (PE)-conjugated Mouse IgG1 isotype control.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Target description
T-cell surface antigen CD2 (UniProt: P06729; also known as Erythrocyte receptor LFA-2, LFA-3 receptor, Rosette receptor, T-cell surface antigen T11/Leu-5, CD2) is encoded by the CD2 (also known as SRBC) gene (Gene ID: 914) in human. CD2 is a transmembrane glycoprotein of the immunoglobulin superfamily expressed on the surface of T cells, NK cells, thymocytes, and dendritic cells. It is a single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-24), which is subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 25-209), a transmembrane domain (aa 210-235), and a cytoplasmic domain (aa 236-351). In its extracellular domain, it contains an Ig-like V-type domain (aa 25-128) and an Ig-Like C2 type domain (aa 129-209). CD2 serves as a receptor for the lymphocyte function-associated antigen CD58 (LFA-3) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. The membrane-proximal domain of its extracellular region connects the adhesion (membrane-distal) domain to the cell membrane and may be involved in CD2 clustering following T cell activation. The membrane-distal domain is responsible for CD58 binding. CD2 is also involved in human T cell development and activation. It plays an important role in the formation and organization of the immunological synapse that is formed between T cells and antigen-presenting cells upon cell-cell conjugation and associated intracellular signaling. Its expression is reported to be upregulated on memory T cells as well as activated T cells. (Ref.: Binder, C., et al. (2020). Front. Immunol. 11; 1090; Moingeon, P., et al. (1989). Immunol. Rev. 111; 111-144).
Physical form
Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted antibody solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.
Reconstitution
1.0 mg/mL after reconstitution at 25 μL in PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL of PBS. Protect from light.
ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany

Quality Level	200
biological source	mouse
antibody form	purified antibody
antibody product type	primary antibodies
clone	TS1/8, monoclonal
product line	ColorWheel®
form	lyophilized
mol wt	calculated mol wt 39.49 kDa
species reactivity	human
packaging	antibody small pack of 25 µL
application(s)	flow cytometry: suitable
isotype	IgG1
epitope sequence	Unknown
Protein ID accession no.	NP_001315538.1 ,
UniProt accession no.	P06729,
shipped in	ambient
storage temp.	2-8°C
Gene Information	human ... CD2(914)