Anti-Human CD138 (MI15) ColorWheel® Dye-Ready mAb CWA-1044-25UL

Specificity
Clone MI15 is a mouse monoclonal antibody that detects Syndecan-1 (CD138). It targets an epitope within the extracellular domain.
A mixture of U266 and XG-1 human myeloma cells.
Quality Control Testing:
Evaluated by Flow Cytometry in U266 cells.
Staining of one million human U266 cells was performed using 5 µL of a 1:1 mixture of Cat. No. CWA-1044, Anti-Human CD138 (MI15) ColorWheel Dye-Ready mAb and Cat. No. CWD-PE Colorwheel® Antibody-Ready Phycoerythrin (PE) Dye or the equivalent amount of Cat. No. CWA-1013 Mouse IgG1 isotype control and Cat. No. CWD-PE Colorwheel® Antibody-Ready Phycoerythrin (PE) Dye.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-Human CD138 (MI15) ColorWheel<® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any Colorwheel® antibody with any Colorwheel® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each Colorwheel® antibody and dye is lyophilized for long long-term storage, allowing for simplicity and flexibility without compromise
Target description
Syndecan-1 (UniProt: P18827; also known as SYND1, CD138) is encoded by the SDC1 (also known as SDC) gene (Gene ID: 6382) in human. Syndecans are transmembrane proteoglycans (PGs) composed of a core protein to which growth factor binding glycosaminoglycan (GAG) side chains are attached. Four members of the Syndecan family have been studied and they include syndecan-1 that is mainly expressed in epithelial cells, syndecan-2 (fibroglycan, SDC2) is present on cells of mesenchymal origin, syndecan-3 (N-syndecan, SDC3) is found in neural tissue and cartilage, and syndecan-4 (amphiglycan, ryudocan, SDC4) is ubiquitously expressed. Syndecans are usually present on the cell surface, but they can also be released by the action of sheddases or accumulate in the cell nucleus and may also be present in body fluids. Syndecan-1 is a single-pass type I membrane protein that is produced with a signal peptide (aa 1-22), which is subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 23-254), a transmembrane domain (aa 255-275), and a cytoplasmic domain (aa 276-310). It is a cell surface proteoglycan that bears both heparan sulfate and chondroitin sulfate and that links the cytoskeleton to the interstitial matrix. It plays a role in cell adhesion, motility, invasion, and intracellular signaling. Syndecan-1 is reported to perform these diverse functions by acting as a co-receptor for a variety of growth factor receptors or as a binding partner to integrins. Its shedding from cell surface is regulated by various matrix metalloproteinases (MMPs), including MMP-7, MMP-9, membrane-bound metalloproteinases (MT-MMP1), and by ADAM10 and ADAM17. (Ref.: Szatmari, T., and Dobra, K. (2013). Front. Oncol. 3: 310; Ramani, VC., et al. (2012). J. Biol. Chem. 287(13); 9952-9961).
Physical form
Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted antibody solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.
Reconstitution
1.0 mg/mL after reconstitution at 25 μL in PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL of PBS. Protect from light.
ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany

Quality Level	200
biological source	mouse
antibody form	purified antibody
antibody product type	primary antibodies
clone	MI15, monoclonal
product line	ColorWheel®
form	lyophilized
mol wt	calculated mol wt 32.46 kDa
species reactivity	human
species reactivity (predicted by homology)	nonhuman primates, rat
packaging	antibody small pack of 25 µL
application(s)	flow cytometry: suitable
isotype	IgG1
epitope sequence	Extracellular domain
Protein ID accession no.	NP_001006947.2,
UniProt accession no.	P18827,
shipped in	ambient
storage temp.	2-8°C
Gene Information	human ... SDC1;SDC(6382)