Anti-Human CD11c (3.9) ColorWheel® Dye-Ready mAb CWA-1056-25UL

Specificity
Clone 3.9 is a mouse monoclonal antibody that detects Integrin alpha-X (CD11c). It targets an epitope within the I-domain from the N-terminal half.
Human monocytes and synovial cells.
Anti-Human CD11c (3.9) ColorWheel<® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any Colorwheel® antibody with any Colorwheel® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each Colorwheel® antibody and dye is lyophilized for long long-term storage, allowing for simplicity and flexibility without compromise
Quality Control Testing:
Evaluated by Flow Cytometry in Human human peripheral blood mononuclear cells (PBMC).
Staining of one million human human peripheral blood mononuclear cells (PBMC) was performed using 5 µL of a 1:1 mixture of Cat. No. CWA-1056, Anti-Human CD11c (3.9) Colorwheel® Dye-Ready mAb and Cat. No. CWD-PE Colorwheel® Antibody-Ready Phycoerythrin (PE) Dye or the equivalent amount of PE-conjugated Mouse IgG1 isotype control.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Target description
Integrin alpha-X (UniProt: P20702; also known as CD11 antigen-like family member C; Leu M5; Leukocyte adhesion glycoprotein p150,95 alpha chain; Leukocyte adhesion receptor p150-95; CD11c) is encoded by the ITGAX (also known as CD11C) gene (Gene ID: 3687) in human. CD11c is a single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-19), which is subsequently cleaved off to generate the mature form that contains a long extracellular domain (aa 20-1107), a transmembrane domain (aa 1108-1128), and a cytoplasmic domain (aa 1129-1163). CD11c is predominantly expressed in macrophages, monocytes, and granulocytes and along with integrin b2 it serves as a receptor for fibrinogen. It recognizes the sequence G-P-R in fibrinogen. The aXb2 heterodimer binds many ligands, including collagen type 1, iC3b, fibrinogen, ICAM-1, and lipopolysaccharide. It is reported to mediate cell-cell interaction during inflammatory responses and is especially important in monocyte adhesion and chemotaxis. The I domains of CD11 are responsible for the binding of ligands and have a unique structure with 6-7 a-helixes and 6b sheets with interconnecting loops. In CD11c the loops on the I domain are involved in fibrinogen binding and loops a3-a4 and bD-a5 are reported to be more important for recognition of fibrinogen. (Ref.: Thomas Vorup-Jensen, T., et al. (2003). Proc. Natl. Acad. Sci. USA. 100(4); 1873-1878; Choi, J., and Nham, S-U. (2002). Biochem. Biophys. Res. Commun. 292(3); 756-760).
Physical form
Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted antibody solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.
Reconstitution
1.0 mg/mL after reconstitution at 25 μL in PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL of PBS. Protect from light.
ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany

Quality Level	200
biological source	mouse
antibody form	purified antibody
antibody product type	primary antibodies
clone	3.9, monoclonal
product line	ColorWheel®
form	lyophilized
mol wt	calculated mol wt 127.38 kDa
species reactivity	human
species reactivity (predicted by homology)	nonhuman primates
packaging	antibody small pack of 25 µL
application(s)	flow cytometry: suitable
isotype	IgG1
epitope sequence	Unknown
Protein ID accession no.	NP_001273304.1,
UniProt accession no.	P20702,
shipped in	ambient
storage temp.	2-8°C
Gene Information	human ... ITGAX;CD11C(3687)