Anti-HSD3B1 Antibody, clone 4C1

eCl@ss:	32160702
NACRES:	NA.41

Кат. номер

MABS2068-25UG

MABS2068-100UG

3 beta-hydroxysteroid dehydrogenase/Delta 54-isomerase type 1 (UniProt: P14060; also known as 3 beta-hydroxysteroid dehydrogenase/Delta 54-isomerase type I, 3-beta-HSD I, Trophoblast antigen FDO161G) is encoded by the HSD3B1 (also known as 3BH, HSDB3A) gene (Gene ID: 3283) in human. HSD3B1 is a single -pass membrane protein localized in mitochondria and endoplasmic reticulum. Its transmembrane region is localized to amino acids 288-308. It is a bifunctional enzyme that catalyzes the oxidative conversion of Delta 5-ene-3-beta-hydroxy steroid and causes oxidative conversion of ketosteroids. It plays a vital role in the biosynthesis of all classes of hormonal steroids. Humans have two HSD3B isozymes. HSD3B1 is mainly expressed in the placenta, mammary and, prostate glands, and other peripheral tissues including the skin and adipose tissue and HSD3B2 is expressed primarily in the adrenal and gonads. They exhibit about 93% sequence homology. (Ref.: Gomez-Sanchez, CE et al. (2017). Steroids 127; 56-61).
Specificity
Clone 4C1 detects 3-beta-HSD I (HSD3B1) in human tissues. It targets an epitope with in 14 amino acids from the N-terminal region.
A KLH-conjugated linear peptide corresponding to 14 amino acids from the N-terminal region of human 3 beta-hydroxysteroid dehydrogenase/Delta 54-isomerase type 1 (HSD3B1).
Epitope: N-terminus
Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected HSD3B1 in human adrenal gland tissue sections.
ELISA Analysis : A representative lot detected HSD3B1 in ELISA applications (Gomez-Sanchez, C.E., et. al. (2017). Steroids. 127:56-61).
Western Blotting Analysis: 1 µg/mL from a representative lot detected HSD3B1 in human placenta tissue lysate in Western blotting applications.
Immunohistochemistry (Paraffin) Analysis: A representative lot detected HSD3B1 in Immunohistochemistry applications (Gomez-Sanchez, C.E., et. al. (2017). Steroids. 127:56-61).
Western Blotting Analysis: A representative lot detected HSD3B1 in Western Blotting applications (Gomez-Sanchez, C.E., et. al. (2017). Steroids. 127:56-61).
Research Category
Signaling
Anti-HSD3B1, clone 4C1, Cat. No. MABS2068, is a mouse monoclonal antibody that detects 3-beta-HSD I (HSD3B1) and has been tested for use in ELISA, Immunohistochemistry (Paraffin), and Western Blotting.
Quality
Evaluated by Immunohistochemistry (Paraffin) in human placenta tissue sections.
Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution of this antibody detected HSD3B1 in human placenta tissue sections.
Target description
~38 kDa observed; 42.25 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physical form
Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G purified
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	mouse
antibody form	purified immunoglobulin
antibody product type	primary antibodies
clone	4C1, monoclonal
species reactivity	human
packaging	antibody small pack of 25 µg
application(s)	ELISA: suitable","immunohistochemistry: suitable (paraffin)","western blot: suitable
isotype	IgG2b
NCBI accession no.	NP_000853.1,
UniProt accession no.	P14060,
Gene Information	human ... HSD3B1(3283)

WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable