Anti-HPa3 Antibody, clone HIC3-2D12

eCl@ss:

32160702

Кат. номер

MABS1998-25UG

MABS1998-100UG

Pancreas performs both the endocrine and the exocrine function. The bulk of pancreas is composed of exocrine cells that produce digestion enzymes which are released into ducts to be emptied into the duodenum. The endocrine pancreas is composed of islets of Langerhans that produce hormones. At least five distinct endocrine cell types have been identified in the endocrine pancreas, each producing a different hormone. Beta cells produce insulin, which is considered as an anabolic hormone and regulates blood glucose levels and is also involved in fat and protein synthesis. Alpha cells produce glucagon that acts when blood glucose levels are low. Ductal cells of pancreas form the epithelial lining of the branched tubes that deliver digestive enzymes into the duodenum. They also secrete bicarbonate to neutralize acidic contents coming in from stomach. Duct cells may contribute up to 10% of all pancreatic cells. Acinar cells function as a unit of the exocrine pancreas and they synthesize, store, and secrete digestive enzymes (lipase, alpha amylase, trypsin, and chymotrypsin), which under normal physiological conditions are activated only after reaching the duodenum. Premature activation of these enzymes within pancreatic acinar cells can lead to acute pancreatitis. Clone HIC-2D2 can be used to mark all non-beta endocrine cell types in human pancreatic tissue. (Ref.: Dorrell, C., et al. (2008). Stem Cell Research (2008). 1(3); 183-194; Grapin-Botton, A (2005). Int. J. Biochem. Cell Biol. 37(3); 504-510; Leung, PS (2006). Int. J. Biochem. Cell Biol. 38(7); 1024-1030).
Specificity
Clone HIC3-2D12 detects human pancreatic non-beta endocrine cells.
Human pancreatic islets enriched in non-beta endocine cells.
Anti-HPa3, clone HIC3-2D12, Cat. No. MABS1998, is a mouse monoclonal antibody that detects human pancreatic non-beta endocrine cells and has been tested for use in Flow Cytometry and Immunohistochemistry (Paraffin).
Research Category
Signaling
Flow Cytometry Analysis: A representative lot detected HPa3 in Flow Cytometry applications (Dorrell, C., et. al. (2008). Stem Cell Res. 1(3):183-94; Bramswig, N.C., et. al. (2013). J Clin Invest. 123(3):1275-84; Dorrell, C., et. al. (2016). Nat Commun. 7:11756).
Quality
Evaluated by Immunohistochemistry (Paraffin) in human pancreatic tissue sections.
Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution of this antibody detected HPa3 in human pancreatic tissue sections.
Physical form
Purified mouse monoclonal antibody IgM in PBS with 0.05% sodium azide.
Format: Purified
Purified by ion-exchange chromatography
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	mouse
antibody form	purified immunoglobulin
antibody product type	primary antibodies
clone	HIC3-2D12, monoclonal
species reactivity	human
packaging	antibody small pack of 25 µg
application(s)	flow cytometry: suitable","immunohistochemistry: suitable (paraffin)
isotype	IgM
NCBI accession no.	NP_004608.1,
UniProt accession no.	P48230,