Anti-HDAC1 Antibody

eCl@ss:	32160702
NACRES:	NA.41

Кат. номер

6-720

6-720-25UG

6-720-100UG

Mammalian HADC1 (also designated HD1), HDAC2 (also designated mammalian RPD3) and HDAC3, all are related to the yeast transcriptional regulator Rpd3p, are identified as histone deacetylases. The deacetylation of histones is associated with transcriptional silencing.
Specificity
Oher species cross-reactivity not tested.
Recognizes and is specific for HDAC1, Mr 65 kDa. May detects a non-specific protein at ~35 kDa.
6X-His-tagged fragment representing amino acids 53-482 of mouse histone deacetylase 1 (HDAC1).
Immunoprecipitation:
5 µg of a previous lot immunoprecipitated HDAC1 from 500 µg of 3T3/A31 RIPA lysate.
Immunocytochemistry:
This antibody has been reported to immunostain HDAC1 in 1% paraformaldehyde fixed, 0.1% Triton X-100 permeabilized 3T3 cells.
HDAC Activity:
This antibody has been reported to immunoprecipitate active HDAC.
Research Sub Category
Histones
Use Anti-HDAC1 Antibody (Rabbit Polyclonal Antibody) validated in EA, ICC, IP, WB to detect HDAC1 also known as histone deacetylase 1.
Research Category
Epigenetics & Nuclear Function
Quality
Routinely evaluated by western blot on RIPA lysates from 3T3/A31 cells.
Western Blot Analysis:
0.5-2 µg/mL of this lot detected HDAC1 in RIPA lysates from 3T3/A31 cells.
Target description
65 kDa
Physical form
Purified rabbit polyclonal IgG in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, containing 0.05% sodium azide. Frozen at -20ºC.
Format: Purified
Protein A chromatography
Storage and Stability
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Control
NIH/3T3 cell extract
Included Positive Antigen Control: Catalog # 12-305, 3T3/A31 Cell Lysate. Add 2.5 µL of 2-mercaptoethanol per 100 µL of lysate and boil for 5 minutes to reduce the preparation. Load 20 µg of reduced lysate per lane for minigels.
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit
Quality Level	100
antibody form	purified immunoglobulin
antibody product type	primary antibodies
clone	polyclonal
species reactivity	human, mouse, rat
packaging	antibody small pack of 25 µg
Торговая марка	Upstate®
application(s)	activity assay: suitable","immunocytochemistry: suitable","immunoprecipitation (IP): suitable","western blot: suitable
isotype	IgG
NCBI accession no.	NP_004955,
UniProt accession no.	Q13547,
shipped in	ambient