Anti-Golgi Zone Antibody, clone 371-4

Кат. номер

MAB1271-I-25UG

MAB1271-I-100UG

Golgi apparatus protein 1 (UniProt: Q92896; also known as CFR-1, Cysteine-rich fibroblast growth factor receptor, E-selectin ligand 1, ESL-1, Golgi sialoglycoprotein MG-160) is encoded by the GLG1 (also known as CFR1, ESL1, MG160) gene (Gene ID: 2734) in human. The Golgi apparatus plays a major role in transport, processing, and sorting of thousands of macromolecules in eukaryotic cells. It is readily recognizable because of its stacked membrane-coated cavities known as dictyosomes. The Golgi complex consists of a stack of flattened, membrane-bound sacs known as cisternae, with three functional regions: the cis face, medial region and trans face and each of these regions contain various enzymes to selectively modify the macromolecules that pass through this complex. The Golgi complex is localized in the vicinity of the nucleus and is usually very large in secretory cells, where it fills most of the cytoplasm. Clone 371-4 selectively recognizes the Golgi complex in human cells. It produces a diffuse staining pattern of the Golgi zone in normal and malignant cells.
Specificity
Clone 371-4 is a mouse monoclonal antibody that detects Golgi apparatus.
SU-DHL-1 human large cell lymphoma cells.
Anti-Golgi Zone, clone 371-4, Cat. No. MAB1271-I, is a mouse monoclonal antibody that detects Golgi apparatus protein 1 and is tested for use in Immunocytochemistry.
Quality Control Testing
Evaluated by Immunocytochemistry in HeLa cells.
Immunocytochemistry Analysis: A 1:500 dilution of this antibody detected Golgi apparatus protein 1 in HeLa cells.
Tested Application
Immunocytochemistry Analysis: A 1:500 dilution of this antibody detected Golgi apparatus protein 1 in A431cells.
Immunocytochemistry Analysis: A representative lot detected Golgi Zone in Immunocytochemistry applications (Endo, Y., et al. (2007). Cancer Sci.;98(10):1633-7; Strehlow, A.N., et al. (2007). Hum Mol Genet.;16(4):391-409; Oiso, S., et al. (2009). J Neurochem.;109(1):158-67; Leite, C., et al. (2014). PLoS One.;9(10):e111059).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Physical form
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality Level	200
biological source	mouse
antibody form	purified antibody
antibody product type	primary antibodies
clone	371-4, monoclonal
mol wt	calculated mol wt 134.55 kDa
purified by	using protein G
species reactivity	human
species reactivity (predicted by homology)	monkey
packaging	antibody small pack of 100 µg
application(s)	immunocytochemistry: suitable
isotype	IgG1
epitope sequence	Unknown
conjugate	unconjugated
Protein ID accession no.	NP_001139138,
UniProt accession no.	Q92896,
shipped in	2-8°C
Gene Information	human ... GLG1(2734)