Anti-GLUT1 (CT) Antibody, clone 5B12.3

eCl@ss:	32160702
NACRES:	NA.41

Кат. номер

MABS132-25UL

MABS132

MABS132-100UL

GLUTs (Glucose transporters) are a family of integral membrane proteins that facilitative glucose uptake by cells. They are integral membrane glycoproteins involved in transporting glucose into most cells. Seven types of glucose transport carrier proteins, designated as GLUT 1 to 7, facilitate glucose transport across the cell membrane. Molecular cloning of glucose transporters have identified a family of closely related genes that encode at least 7 proteins exhibiting high degree of amino acid homology (45% to 65%), all in the molecular weight range of 40 to 60 kDa. GLUT1 functions in constitutive or basal glucose uptake in adult tissues and is the predominant glucose transporter in embryonic and fetal tissues. It has two forms, a 55 kDa and a 45 kDa. The 55 kDa form of GLUT1 regulates import of glucose from blood to brain across the endothelial cells of the blood-brain barrier (BBB), whereas the 45 kDa form of GLUT1 predominantly regulates nonvascular glial glucose uptake.
Specificity
This antibody recognizes GLUT1 at the C-terminus.
Epitope: C-terminus
KLH-conjugated linear peptide corresponding to the C-terminus of human GLUT1.
Anti-GLUT1 (C-terminus) Antibody, clone 5B12.3 is an antibody against GLUT1 (C-terminus) for use in Western Blotting & IHC.
Research Category
Signaling
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected GLUT1 in rat cerebellum and human cerebellum tissues.
Research Sub Category
Developmental Signaling
Quality
Evaluated by Western Blot in NGF treated PC-12 cell lysate.
Western Blot Analysis: 0.1 µg/mL of this antibody detected GLUT1 in 10 µg of NGF treated PC-12 cell lysate.
Target description
~54 kDa observed
Physical form
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
NGF treated PC-12 cell lysate
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality Level	100
biological source	mouse
antibody form	purified immunoglobulin
antibody product type	primary antibodies
clone	5B12.3, monoclonal
species reactivity	rat, human
packaging	antibody small pack of 25 µL
application(s)	immunohistochemistry: suitable","western blot: suitable
isotype	IgG1
NCBI accession no.	NP_006507,
UniProt accession no.	P11166,
shipped in	ambient
storage temp.	2-8°C
Gene Information	human ... SLC2A1(6513)

WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable