Anti-GCP2 Antibody, clone GCP2-02

eCl@ss:

32160702

Кат. номер

MABT1322-25UG

MABT1322-100UG

Gamma-tubulin complex component 2 (UniProt: Q921G8; also known as GCP2) is encoded by the Tubgcp2 (also known as Gcp2) gene (Gene ID: 74237) in murine species. Gamma-tubulin complex that is essential for microtubule nucleation at the centrosome is composed of gamma-tubulin, GCP2, GCP3, GCP4, GCP5 and GCP6. GCPs are characterized by sequence homology in two specific regions known as the grip1 and grip2 motifs. GPCs associate with gamma-tubulin to form a V-shaped subcomplex known as gamma-tubulin small complex. In this complex GCPs 2 and 3 interact via their N-terminal domain and form the arms of the V. The two-distal part of the V (C-terminal domains) bind to gamma-tubulin. The gamma-tubulin complex serves as a template for polarized growth and regulation of microtubules in eukaryotes. Significantly higher expression of both GPC2 and 3 have been reported in glioblastoma cells and they are concentrated I the centrosomes in interphase glioblastoma cells. Depletion of GPC2 and 3 causes accumulation of cells in G2/M and result in mitotic delay. (Ref.: Farache, D., et al. (2016). J. Biol. Chem. 291(44); 23112-25; Draberova, E., et al. (2015). J. Neuropathol. Exp. Neurol. 74(7); 723-42).
Specificity
Clone GCP2-02 specifically detects GCP2 in T98G glioblastoma cells. It targets an epitope with in 12 amino acids from the N-terminal region.
GST-tagged recombinant fragment corresponding to the first 194 amino acids from the N-terminal region of murine Gamma-tubulin complex component 2.
Research Category
Cell Structure
Anti-GCP2, clone GCP2-02, Cat. No. MABT1322, is a mouse monoclonal antibody that detects Gamma-tubulin complex component 2 (CGP2) and has been tested for use in ELISA, Electron Microscopy, Immunocytochemistry, Immunoprecipitation, and Western Blotting.
Western Blotting Analysis: 1 µg/mL from a representative lot detected GCP2 in human glioblastoma T98G cell lysate.
Immunoprecipitation Analysis: A representative lot immunoprecipitated GCP2 in Immunoprecipitation applications (Draberova, E., et. al. (2015). J Neuropathol Exp Neurol. 74(7):723-42).
Immunocytochemistry Analysis: A representative lot detected GCP2 in Immunocytochemistry applications (Draberova, E., et. al. (2015). J Neuropathol Exp Neurol. 74(7):723-42).
Western Blotting Analysis: A representative lot detected GCP2 in Western Blotting applications (Draberova, E., et. al. (2015). J Neuropathol Exp Neurol. 74(7):723-42).
ELISA Analysis: A representative lot detected GCP2 in ELISA applications (Draberova, E., et. al. (2015). J Neuropathol Exp Neurol. 74(7):723-42).
Electron Microscopy Analysis: A representative lot detected GCP2 in Electron Microscopy applications (Draberova, E., et. al. (2015). J Neuropathol Exp Neurol. 74(7):723-42).
Immunoprecipitation Analysis: A represnetative lot immunoprecipitated GCP2 in mouse bone-marrow mast cells (Courtesy of Pavel Draber, Ph.D., Institute of Molecular Genetics of the ASCR, v.v.i., Prague, Czech Republic).
Quality
Evaluated by Western Blotting in T98G human glioblastoma cell lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected GCP2 in lysate from T98G human glioblastoma cells.
Target description
~100 kDa observed; 103.22 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physical form
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G purified
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	mouse
antibody form	purified antibody
antibody product type	primary antibodies
clone	GCP2-02, monoclonal
species reactivity	fish, human, frog, chicken, mouse
packaging	antibody small pack of 25 µg
application(s)	ELISA: suitable","electron microscopy: suitable","immunocytochemistry: suitable","immunoprecipitation (IP): suitable","western blot: suitable
isotype	IgG1
NCBI accession no.	NP_001272936.1,
UniProt accession no.	Q921G8,