Anti-GARP Antibody, clone 4B1

eCl@ss:

32160702

Кат. номер

MABN2429

MABN2429-25UG

Cyclic nucleotide-gated cation channel beta-1 (UniProt: Q14028; also known as Cyclic nucleotide-gated cation channel 4, CNG channel 4, CNG-4, CNG4, Cyclic nucleotide-gated cation channel gamma, Cyclic nucleotide-gated cation channel modulatory subunit, Cyclic nucleotide-gated channel beta-1, CNG channel beta-1, CNGB1, Glutamic acid-rich protein, GARP) is encoded by the CNGBI (also known as CNCG2, CNCG3L, CNCG4, RCNC2) gene in human. CNGB1 is a multi-pass membrane protein that is a subunit of cyclic nucleotide-gated (CNG) channels that are non-selective cation channels and play important roles in both visual and olfactory signal transduction. It forms a tetramer with three CNGA1 subunits. When associated with CNGA1, it is involved in the regulation of ion flow into the rod photoreceptor outer segment (ROS), in response to light-induced alteration of the levels of intracellular cGMP. CNGB1 contains one cyclic nucleotide-binding domain. Four isoforms of CNGB1 have been described that are generated by alternate splicing. Mutations in CNGB1 gene are associated with retinitis pigmentosa that is characterized by retinal pigment deposits that are visible on fundus examination and case loss of rod photoreceptor cells followed by secondary loss of cone photoreceptors.
Specificity
Clone 4B1 detects Glutamic acid-rich protein (GARP) in mouse and bovine retina. It targets an epitope with in the first 16 amino acids from the N-terminal region.
Epitope: N-terminus
GST-tagged first 16 amino acids from the N-terminal region of human Cyclic nucleotide-gated cation channel beta-1.
Anti-GARP, clone 4B1, Cat. No. MABN2429, is a highly specific mouse monoclonal antibody, that targets CNGB1 and has been tested in Immunohistochemistry (Paraffin), Immunoprecipitation, Immunofluorescence and Western Blotting.
Western Blotting Analysis: A representative lot detected GARP in Western Blotting applications (Poetsch, A., et. al. (2001). J Biol Chem. 276(51):48009-16; Chakraborty, D., et. al. (2008). Biochemistry. 47(4):1144-56; Zulliger, R., et. al. (2015). PLoS One. 10(9):e0138508).
Immunofluorescence Analysis: A representative lot detected GARP in Immunofluorescence applications (Zhang, Y., et. al. (2009). J Cell Sci. 122(Pt 8):1192-200).
Immunoprecipitation Analysis: A representative lot detected GARP in Immunoprecipitation applications (Conley, S.M., et. al. (2010). Adv Exp Med Biol. 664:63-70).
Research Category
Neuroscience
Quality
Evaluated by Immunohistochemistry in mouse retina tissue.
Immunohistochemistry Analysis: A 1:1,000 dilution of this antibody detected GARP in mouse retinal tissue.
Target description
139.68 kDa calculated.
Physical form
Format: Purified
Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Protein G purified
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality Level	100
biological source	mouse
antibody form	purified immunoglobulin
antibody product type	primary antibodies
clone	4B1, monoclonal
species reactivity	bovine, mouse
species reactivity (predicted by homology)	rat (based on 100% sequence homology), human (based on 100% sequence homology)
packaging	antibody small pack of 25 µg
application(s)	immunofluorescence: suitable","immunohistochemistry: suitable (paraffin)","immunoprecipitation (IP): suitable","western blot: suitable
isotype	IgG2a
NCBI accession no.	NP_001288,
UniProt accession no.	Q14028,

WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable