Anti-Frizzled-2

eCl@ss:	32160702
NACRES:	NA.41

Кат. номер

ABS1491-25UL

ABS1491-100UL

Frizzled-2 (UniProt: Q14332; also known as Fz-2, hFz2, FzE2) is encoded by the FZD2 gene (Gene ID: 2535) in human. Frizzled-2 is a multi-pass membrane protein that is widely expressed in adult tissues. In fetus, higher levels are expressed in the brain, lung, and kidney and lower levels are observed in liver. Frizzled serves as a receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. In the presence of Wnt the Frizzled receptor is activated, which along with low-density lipoprotein receptor-related proteins (LRP) 5 and 6 stabilizes beta-catenin. The formation of Wnt-Frizzled-LRP6 complex, together with the recruitment of the scaffolding protein Dishevelled (Dvl), results in LRP6 phosphorylation and activation and the recruitment of the Axin complex to the receptors. This leads to the inhibition of Axin-mediated beta-catenin phosphorylation, which stabilizes beta-catenin. Frizzled-2 is synthesized with a signal peptide (aa 1-23), which is subsequently cleaved off in the mature form. Its Fz domain is localized to amino acids 34-153 and this domain is involved in its binding with Wnt ligands. Frizzled-2 can be ubiquitinated by RING-type E3 ubiquitin transferase ZNRF3 leading to its proteasomal degradation. Abnormalities in Wnt signaling are reported to promote birth defects, degenerative diseases, and cancer.
Specificity
This rabbit polyclonal antibody specifically detects human Frizzled -2. It targets an epitope with in 13 amino acids from the extracellular domain.
KLH-conjugated linear peptide corresponding to 13 amino acids from the N-terminal half of human Frizzled-2.
Epitope: unknown
Detects Frizzled-2 using this rabbit polyclonal Anti-Frizzled-2 Antibody, Cat. No. ABS1491, tested for use in Western Blotting.
Research Category
Signaling
Quality
Evaluated by Western Blotting in CACO-2 cell lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected Frizzled-2 in CACO-2 cell lysate.
Target description
~60 kDa observed; 63.55 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physical form
Affinity Purified
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit
antibody form	affinity isolated antibody
antibody product type	primary antibodies
clone	polyclonal
purified by	affinity chromatography
species reactivity	human
species reactivity (predicted by homology)	mouse (based on 100% sequence homology)
packaging	antibody small pack of 25 µL
application(s)	western blot: suitable
UniProt accession no.	Q14332,
Gene Information	human ... FZD2(2535)