Anti-FOXO1 Antibody, clone 2H8.2

eCl@ss:	32160702
NACRES:	NA.41

Кат. номер

5-1075

5-1075-25UG

5-1075-100UG

Forkhead box O (FOXO) transcription factors play an important role in modulating metabolic functions. Given the relatively high expression of FOXO1 in insulin-responsive tissues, this transcription factor is highly poised to regulate energy metabolism. When nutrient and insulin levels are low, FOXO1 promotes expression of gluconeogenic enzymes. Conversely, in the fed state, insulin levels rise and stimulate uptake of glucose primarily into skeletal muscle and other organs, including adipose tissue. Under certain pathophysiologic conditions, including insulin resistance, negative signaling to FOXO1 is compromised. (Gross, D.N., 2009).
Specificity
This antibody detects FOXO1.
FOXO1 shuttles between the nucleus and the cytosol.
GST-tagged recombinant protein corresponding to full length FOXO1.
Research Sub Category
Transcription Factors
Research Category
Epigenetics & Nuclear Function
Use Anti-FOXO1 Antibody, clone 2H8.2 (Mouse Monoclonal Antibody) validated in WB, ICC, IHC(P) to detect FOXO1 also known as Forkhead box protein O1A, Forkhead in rhabdomyosarcoma.
Immunocytochemistry: A 1:500 dilution of a previous lot was used.
Immunocytochemistry: Representative lot data.
A 1:500 dilution of a previous lot was used.
Immunohistochemistry (Paraffin): Representative lot data.
Optimal Staining With Epitope Retrieval: Ductal Carcinoma In Situ.
Quality
Evaluated by western blot on HEK293 cell lysate.
Western Blot Analysis: 2 µg/mL of this antibody detected FOXO1 in HEK293 cell lysate.
Target description
~78-82 kDa
Replaces: 04-1005
Physical form
Protein G Purified
Format: Purified
Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8ºC from date of receipt.
Analysis Note
Control
HEK293 cell lysate
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality Level	100
biological source	mouse
antibody form	purified antibody
antibody product type	primary antibodies
clone	2H8.2, monoclonal
species reactivity	human, rat, mouse
packaging	antibody small pack of 25 µg
application(s)	immunocytochemistry: suitable","immunohistochemistry: suitable (paraffin)","western blot: suitable
isotype	IgG1
NCBI accession no.	NP_002006.2,
UniProt accession no.	Q12778,
shipped in	ambient