Anti-FOXK1 (MNF)

eCl@ss:	32160702
NACRES:	NA.41

Кат. номер

ABE1325

ABE1325-25UG

Forkhead box protein K1 (UniProt: P85037; also known as Myocyte nuclear factor, MNF) is encoded by the FOXK1 (also known as MNF) gene (Gene ID: 221937) in human. Forkhead box protein K1 is a transcriptional regulator that binds to the upstream enhancer region (CCAC box) of myoglobin gene. It is an important regulatory factor of the myogenic progenitor cell population. It is involved in the cell cycle process and promotes proliferation by repressing Foxo4 transcriptional activity and the cyclin-dependent kinase inhibitor, p21CIP, in the myogenic progenitor cells. It is shown to represses myogenic differentiation by inhibiting MEFC activity and has a role in remodeling processes of adult muscles that occur in response to physiological stimuli. It is shown to be essential for correct temporal orchestration of molecular and cellular events necessary for muscle repair. Forkhead box protein K1 is expressed both developing and adult tissues. In adults, significant expression is seen in tumors of the brain, colon, and lymph node. Two isoforms of Forkhead box protein K1 have been reported that are produced by alternative splicing. Both FOXK1 and FOXK2 proteins are shown to act as transcriptional repressors of autophagy in muscle cells and fibroblasts. They specifically recruit Sin3A HDAC complexes to restrict acetylation of histone H4 and expression of critical autophagy genes. The loss of FOXK1 and FOXK2 proteins results in the upregulation of several genes controlling the initiation and nucleation of autophagosome formation. (Ref.: Bowman, CJ., et al. (2014). Nature Cell Biology 16(12); 1202-1214).
Specificity
This rabbit polyclonal antibody detects Forkhead box protein K1 in human cells. It targets an epitope within 20 amino acids from the C-terminal region.
KLH-conjugated linear peptide corresponding to 20 amino acids from the C-terminal region of human Forkhead box protein K1.
Epitope: C-terminus
Western Blotting Analysis: 2 µg/mL from a representative lot detected FOXK1 (MNF) in HepG2 cell lysate.
Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected FOXK1 (MNF) in human cerebral cortex, human liver, and human testis tissues.
Research Category
Epigenetics & Nuclear Function
Western Blotting Analysis: 2 µg/mL from a representative lot detected FOXK1 (MNF) in HepG2 cell lysate.
Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected FOXK1 (MNF) in human cerebral cortex, human liver, and human testis tissues.
Anti-FOXK1 (MNF), Cat. No. ABE1325, is a highly specific rabbit polyclonal antibody that targets Forkhead box protein K1 and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.
Quality
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: 2 µg/mL of this antibody detected FOXK1 (MNF) in HeLa cell lysate.
Target description
~95 kDa observed; 75.46 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physical form
Affinity Purified
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Stable for 1 year at 2-8°C from date of receipt.
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit
Quality Level	100
antibody form	affinity isolated antibody
antibody product type	primary antibodies
clone	polyclonal
purified by	affinity chromatography
species reactivity	human
packaging	antibody small pack of 25 µg
application(s)	immunohistochemistry: suitable (paraffin)","western blot: suitable
isotype	IgG
NCBI accession no.	NP_001032242.1,
UniProt accession no.	P85037,
shipped in	ambient
Gene Information	human ... FOXK1(221937)

Flash Point F	does not flash
Flash Point C	does not flash