Anti-FGFR-4 Antibody, clone 29.2

eCl@ss:

32160702

Кат. номер

MABC1127-25UL

MABC1127-100UL

Fibroblast growth factor receptor 4 (UniProt: P22455; also known as EC: 2.7.10.1, FGFR-4, CD334) is encoded by the FGRF4 (also known as JTK2, TKF) gene (Gene ID: 2264) in human. FGFR-4 is a highly conserved, single-pass type I membrane protein with tyrosine kinase activity that serves as a cell surface receptor for fibroblast growth factors and regulates cell proliferation differentiation, and migration. FGFR-4 is expressed in gastrointestinal epithelial cells, pancreas, and gastric and pancreatic cancer cell lines. In the absence of a ligand, it is present as a monomer in an inactive conformation. Upon ligand binding it homodimerizes into an active conformation with autophosphorylation of tyrosine residues. FGFR-4 is synthesized with a signal peptide (aa 1-21), which is subsequently cleaved off in the mature form. It contains an extracellular domain (aa 22-369), a transmembrane domain (aa 370-390), and a cytoplasmic domain (aa 391-802). FGFR-4 undergoes N-glycosylation, and when not fully glycosylated it can undergo proteasomal degradation. FGFR-4 signaling is down-regulated by receptor internalization and degradation. MMP14 is reported to promote internalization and degradation of FGFR-4. Mutations that lead to constitutive kinase activation or impair normal FGFR-4 inactivation lead to aberrant signaling leading to tumor growth. (Ref.: Turkington, RC et al. (2014). Cell Death Dis. 5; e1046).
Specificity
This rabbit monoclonal antibody detects human fibroblast growth factor receptor 4 (FGFR-4). It targets an epitope with in the extracellular domain.
Epitope: extracellular domain
His-tagged recombinant extracellular domain of human fibroblast growth factor receptor 4 (FGFR-4).
Anti-FGFR-4, clone 29.2, Cat. No. MABC1127, is a rabbit monoclonal antibody that detects Fibroblast growth factor receptor 4 and has been tested for use in Flow Cytometry.
Flow Cytometry Analysis: A 1:4 dilution from a representative lot detected FGFR-4 in one million LNCaP cells.
Research Category
Signaling
Quality
Evaluated by Flow Cytometry in DU-145 cells.
Flow Cytometry Analysis: A 1:4 dilution of this antibody detected FGFR-4 in one million DU-145 cells.
Target description
87.95 kDa calculated.
Physical form
Rabbit monoclonal antibody in supernatant without preservatives.
Unpurified
Storage and Stability
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit
antibody form	culture supernatant
antibody product type	primary antibodies
clone	29.2, monoclonal
species reactivity	human
packaging	antibody small pack of 25 µL
application(s)	flow cytometry: suitable
isotype	IgG
NCBI accession no.	NP_001278909,
UniProt accession no.	P22455,
Gene Information	human ... FGFR4(2264)

WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable