Anti-FGF23 Antibody, clone 278-2

Кат. номер

MABS460-I-25UG

MABS460-I-100UG

Fibroblast growth factor 23 (UniProt: Q9GZV9; also known as FGF-23, Phosphatonin, Tumor-derived hypophosphatemia-inducing factor) is encoded by the FGF23 (also known as HYPF, UNQ3027/PRO9828) gene (Gene ID: 8074) in human. FGF23 is a growth factor that is produced primarily in the bone tissue and its primary hormonal function is to regulate phosphate homeostasis and vitamin D metabolism. Unlike other FGFs, it has a low affinity for heparin sulfate, which allows it to enter the circulation and act as an endocrine hormone. It inhibits renal tubular phosphate transport by reducing sodium-dependent phosphate transport protein 2A (SLC34A1) levels. FGF23 binding to FGFR requires a coreceptor, Klotho, which acts as a scaffold for docking with FGFR1. Together with Klotho, it modulates phosphate reabsorption and both 1 hydroxylation and 24hydroxylation in the renal proximal tubules. FGF23 is reported to inhibit the expression of 1 hydroxylase while increasing the expression of 24hydroxylase, thus reducing the concentration of active 1,25(OH)2D. It can also act directly on the parathyroid to reduce parathyroid hormone secretion. (Ref.: Almel, EA., et al. (2019). J. Bone Min. Res (JMBR Plus). 3(8): e10190).
Specificity
Clone 278.2 is a mouse monoclonal antibody that detects human Fibroblast growth factor 23 (FGF-23).
Recombinant human Fibroblast growth factor 23 (FGF23).
Anti-FGF23, clone 278-2, Cat. No. MABS460-I, is a mouse monoclonal antibody that detects Fibroblast growth factor 23 and is tested for use in Flow Cytometry and Western Blotting.
Quality Control Testing
Evaluated by Western Blotting in Human brain tissue lysates.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected FGF23 in Human brain tissue lysates.
Tested Applications:
Flow Cytometry Analysis (FC): 1 µg of this antibody detected Fibroblast growth factor 23 (FGF23) in one million Jurkat cells pretreated with Brefeldin A (1 µg/mL 4 h).
Physical form
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality Level	200
biological source	human
antibody form	purified antibody
antibody product type	primary antibodies
clone	278-2, monoclonal
mol wt	calculated mol wt 27.95 kDa","observed mol wt ~37 kDa
purified by	using protein G
species reactivity	human
packaging	antibody small pack of 100 µg
application(s)	flow cytometry: suitable","western blot: suitable
isotype	IgG1
epitope sequence	Unknown
conjugate	unconjugated
Protein ID accession no.	NP_065689,
UniProt accession no.	Q9GZV9,
shipped in	2-8°C
Gene Information	human ... FGF23(8074)