- Производитель:
- Sigma-Aldrich
Кат. номер |
SAB4200836-25UL |
SAB4200836-100UL |
Specificity
Anti-FAT10 antibody specifically recognizes FAT10 from human origin.
Biochem/physiol Actions
FAT10 is a unique UBL containing a C-terminal GG motif available for activation and conjugation. This motif differentiates FAT10 from ubiquitin and other UBLs the needs to be cleaved from precursors. Furthermore, unlike ubiquitin that requires a poly-ubiquitin chain, the FAT10 can bind the 26S proteasome as a monomer to mediate proteasomal degradation.2-3 Under normal conditions, FAT10 have short half-life, its expression is repressed by p534 and is restricted to tissues of the immune system like thymus, lymph nodes and the spleen. Inflammatory cytokines such as IFN? and TNFa and downstream STAT3 and NF-?B signaling can induce its expression also in other tissues.2-3,5 FAT10 expression is also upregulated and can be increased up to 100-fold in many cancer types including liver and colon cancer HCC, colorectal, ovarian and uterine carcinomas triple-negative breast cancer, bladder cancer, gastric cancer, glioma, pancreatic ductal, adenocarcinoma and osteosarcoma.2,5-6 FATylation has an important role in many cellular process varied from cell maintenance to DNA repair. Understanding FAT10 mechanism and finding its downstream substrates is important to reveal its role in tumorigenesis.1-7
Physical form
Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative.
Storage and Stability
For continuous use, store at 2-8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
biological source | rabbit |
antibody form | affinity isolated antibody |
antibody product type | primary antibodies |
clone | polyclonal |
form | buffered aqueous solution |
packaging | antibody small pack of 25 µL |
concentration | ~1 mg/mL |
application(s) | immunoblotting: 1:1000-1:2000 using human HepG2 treated with TNF-& IFNG& MG-132 whole cell extract. |
UniProt accession no. | O15205 , |
shipped in | dry ice |
storage temp. | 20°C |
Gene Information | human ... UBD(10537) |
Flash Point F | Not applicable |
Flash Point C | Not applicable |
Получите коммерческое предложение в течение 1 часа
Менеджер подготовит коммерческое предложение и позвонит, если понадобится уточнить детали вашего заказа

С 2010 года мы поставляем оборудование с заводов Европы. Берем на себя все — от подбора оборудования до внедрения на предприятии

Все сотрудники имеют высшее образование, закончили ведущие химические вузы страны, такие как РХТУ им Менделеева.

У большинства компаний срок ожидания составляет 10-12 недель.

Оборудование хранится на сухом отапливаемом складе, где поддерживается ровная температура.

Работаем с PonyExpress и Деловыми линиями. Вы также можете выбрать свою транспортную компанию или забрать товар со склада в Москве.

В случае любых неполадок за свой счет выполним ремонт в сервисном центре или на заводе-изготовителе. Или бесплатно заменим прибор на новый.

Производим пуско-наладку оборудования, валидацию, обучение сотрудников. Если нужно, привлекаем инженеров с заводов- изготовителей.