Anti-FANCD2 Antibody, clone 10F8.1

eCl@ss:

32160702

Кат. номер

MABS1889-100UG

MABS1889-25UG

Fanconi anemia group D2 protein (UniProt: Q9BXW9; also known as Protein FACD2) is encoded by the FANCD2 (also known as FACD) gene (gene ID: 2177) in human. FACD2 is a nuclear protein that is required for maintenance of chromosomal stability. It is highly expressed in fetal oocytes, and in hematopoietic cells of the fetal liver and bone marrow. High expression is also observed in germinal center cells of the spleen, tonsil, and reactive lymph nodes and in the proliferating basal layer of squamous epithelium of tonsil, esophagus, larynx and cervix. FACD2 promotes accurate and efficient pairing of homologs during meiosis. It is also involved in the repair of DNA double-strand breaks, both by homologous recombination and single-strand annealing and may participate in S phase and G2 phase checkpoint activation upon DNA damage. It is concentrated in nuclear foci during S phase and upon genotoxic stress. FACD2 plays a role in preventing breakage and loss of missegregating chromatin at the end of cell division, particularly after replication stress. It is phosphorylated in response to various genotoxic stresses by ATM and/or ATR. Upon ionizing radiation, it is phosphorylated on serine 222 and serine 1404 by ATM. Phosphorylation on serine 222 is shown to be essential for S-phase checkpoint activation. Mutations in FANCD2 gene are known to cause Fanconi anemia complementation group D2, which results in anemia, leukopenia, and thrombopenia and may cause cardiac, renal, and limb malformations and increase predisposition to malignancies. (Ref.: Shen, Y., et al. (2015). Cancer Cell Microenviron. 2(4): e986).
Specificity
Clone 10F8.1 specifically detects human Fanconi anemia group D2 protein. It targets an epitope with in the first 150 amino acids from the N-terminal region.
GST/His-tagged recombinant fragment corresponding to first 150 amino acids of human Fanconi anemia group D2 protein.
Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected FANCD2 in human tonsil and human testis tissue sections.
Research Category
Signaling
Anti-FANCD2, clone 10F8.1, Cat. No. MABS1889, is a mouse monoclonal antibody that detects Fanconi anemia group D2 protein and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
Quality
Evaluated by Western Blotting in Jurkat cell lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected FANCD2 in Jurkat cell lysate.
Target description
~166 kDa observed; 164.13 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physical form
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G purified
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	mouse
antibody form	purified immunoglobulin
antibody product type	primary antibodies
clone	10F8.1, monoclonal
species reactivity	human
packaging	antibody small pack of 25 µg
application(s)	immunohistochemistry: suitable (paraffin)","western blot: suitable
isotype	IgG1
NCBI accession no.	NP_149075,
UniProt accession no.	Q9BXW9,

WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable