Anti-Factor XII Antibody, clone 9A2

eCl@ss:	32160702
NACRES:	NA.41

Кат. номер

MABS2050-25UG

MABS2050-100UG

Coagulation factor XII (UniProt: P00748; also known as EC: 3.4.21.38, Hageman factor, HAF) is encoded by the F12 gene (Gene ID: 2161) in human. Factor XII is a serum glycoprotein that participates in the initiation of blood coagulation, fibrinolysis, and the generation of bradykinin and angiotensin. It activates factor XI to XIa and factor VII to VIIa by selective cleavage of Arginine-Isoleucine bond. Factor XII itself can be activated to factor XIIa either by exposure to negatively charged surfaces or via proteolytic cleavage. It is synthesized in the liver with a signal peptide (aa 1-19), which is subsequently cleaved off in the mature form. Coagulation factor XII circulates in blood as a single chain zymogen, which is converted to a two-chain serine protease with a heavy chain (alpha-factor XIIa) and a light chain. The heavy chain contains two fibronectin-type domains, two epidermal growth factor (EGF)-like domains, a kringle domain, and a proline-rich domain. The light chain contains only a catalytic domain. Mutations in F12 gene can lead to Factor XII deficiency that is generally an asymptomatic anomaly of in vitro blood coagulation. However, some mutations may cause hereditary angioedema 3, which is characterized by episodic local swelling involving subcutaneous or submucous tissue of the upper respiratory and gastrointestinal tracts, face, extremities, and genitalia.
Specificity
Clone 9A2 is a mouse monoclonal antibody that specifically detects Coagulation factor XII.
A mixture of purified human Factor XII and Factor XIIa.
ELISA Analysis: A representative lot detected Factor XII in ELISA applications (Matafonov, A., et. al. (2014). Blood. 123(11):1739-46).
Western Blotting Analysis: A representative lot detected Factor XII in Western Blotting applications (Matafonov, A., et. al. (2014). Blood. 123(11):1739-46).
Inhibition Analysis: A representative lot inhibited the generation of thrombin in normal plasma following addition of activated partial thromboplastin reagent. (Matafonov, A., et. al. (2014). Blood. 123(11):1739-46).
Function Analysis: A representative lot prolong the activated partial thromboplastin time. (Matafonov, A., et. al. (2014). Blood. 123(11):1739-46).
Research Category
Signaling
Anti-Factor XII, clone 9A2, Cat. No. MABS2050, is a mouse monoclonal antibody that detects Coagulation factor XII and has been tested for use in ELISA, Inhibition and Function assay, and Western Blotting.
Quality
Evaluated by Western Blotting in human factor XII.
Western Blotting Analysis: 4 µg/mL of this antibody detected Factor XII in human factor XII.
Target description
~75 kDa observed; 67.79 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physical form
Format: Purified
Purified mouse monoclonal antibody IgG1 in PBS, pH 7.4, without preservatives.
Protein G purified
Storage and Stability
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	mouse
antibody form	purified immunoglobulin
antibody product type	primary antibodies
clone	9A2, monoclonal
species reactivity	human, baboon
packaging	antibody small pack of 25 µg
application(s)	ELISA: suitable","western blot: suitable
isotype	IgG1
NCBI accession no.	NP_000496,
UniProt accession no.	P00748,