Anti-Exo1

eCl@ss:

32160702

Кат. номер

ABE1354-25UG

ABE1354-100UG

Exonuclease 1 (UniProt: Q9UQ84; also known as hExo1, Exonuclease I, hExoI) is encoded by the EXO1 (also known as EXOI, HEX1) gene (Gene ID: 9156) in human. Exonuclease 1 is a magnesium-dependent protein with 5 to 3 exonuclease and an RNase H activity. It may also possess a cryptic 35 double-stranded DNA exonuclease activity. It is highly expressed in bone marrow, testis and thymus. Higher levels of expression are also observed in fetal liver. Lower expression has been observed in colon, lymph nodes, ovary, placenta, prostate, small intestine, spleen, and stomach. It functions in DNA mismatch repair to excise mismatch-containing DNA tracts directed by strand breaks located either 5 or 3 to the mismatch. It interacts physically with the DNA mismatch repair proteins, Msh2 and Mlh1, and is involved in the excision of the mispaired nucleotide. Exonuclease 1 is phosphorylated upon DNA damage and in response to agents stalling DNA replication, probably by ATM or ATR. It is shown to be phosphorylated at Serine 454, Threonine 621, and Serine 714 upon DNA-damage caused by treatment with hydroxyurea, but not upon IR treatment. The hydroxyurea-induced triple phosphorylation of exonuclease 1 facilitates destabilization/degradation of the protein.
Specificity
This rabbit polyclonal antibody detects Exonuclease 1 in human cells. It targets an epitope within 16 amino acids from the C-terminal half.
KLH-conjugted linear peptide corresponding to 16 amino acids from the C-terminal half of human Exonuclease 1. The immunogen sequence is conserved in both isoforms 1 and 2.
Epitope: unknown
Immunoprecipitation Analysis: A representative lot detected Exo1 in HeLa cell lysate (Courtesy of Dr. Zhongsheng You at Washington University in St. Louis).
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Exo1 in HeLa and siExo1 hela cell lysates (Courtesy of Dr. Zhongsheng You at Washington University in St. Louis).
Anti-Exo1, Cat. No. ABE1354, is a highly specific rabbit polyclonal antibody that targets Exonuclease 1 and has been tested for use in Immunoprecipitation and Western Blotting.
Research Category
Epigenetics & Nuclear Function
Quality
Evaluated by Western Blotting in 293T cells transfected with GFP-Exo1.
Western Blotting Analysis: 2 µg/mL of this antibody detected Exo1 in 293T cells transfected with GFP-Exo1.
Target description
~145 kDa observed; 94.10 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physical form
Affinity Purified
Format: Purified
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit
Quality Level	100
antibody form	affinity isolated antibody
antibody product type	primary antibodies
clone	polyclonal
species reactivity	human
packaging	antibody small pack of 25 µg
application(s)	immunoprecipitation (IP): suitable","western blot: suitable
NCBI accession no.	NP_006018,
UniProt accession no.	Q9UQ84,
Gene Information	human ... EXO1(9156)