Anti-EWS Antibody, clone 21B1

eCl@ss:

32160702

Кат. номер

MABE1932-25UL

MABE1932-100UL

RNA-binding protein EWS (UniProt: Q01844; also known as EWS oncogene, Ewing sarcoma breakpoint region 1 protein) is encoded by the EWSR1 (also known as EWS) gene (Gene ID: 2130) in human. EWS is member of the TET family of DNA and RNA-binding proteins, which also includes translocated in liposarcoma/fused in sarcoma protein (FUS/TLS) and TATA-binding protein-associated factor 15. It is a multifaceted RNA binding protein with established roles in transcription, pre-mRNA processing, and DNA damage response. It contains a transcriptional-activation domain (EAD), 3 glycine-arginine rich regions, an RNA-binding domain, and a zinc finger domain (aa 518-549). EWS is highly methylated on arginine residues and methylation is mediated by PRMT1 and to some extent by PRMT8. Various environmental signals are known to induce post-translational modifications in its RNA binding domain and glycine-arginine rich domains, thus modulating EWS activity. EWS can relocate from cytoplasm to ribosomes upon PTK2B/FAK2 activation. Mutations in EWS gene are known to cause Ewing sarcoma, a highly malignant, metastatic, primitive small round cell tumor of bone and soft tissue.
Specificity
Clone 21B1 is a rat monoclonal antibody that detects human RNA-binding protein EWS. It targets an epitope with in 20 amino acids from the C-terminal region.
Ovalbumin-conjugated linear peptide corresponding to 20 amino acids from the RGG3 domain of human RNA-binding protein EWS.
Anti-EWS, clone 21B1, Cat. No. MABE1932, is a highly specific rat monoclonal antibody that targets RNA-binding protein EWS and has been tested in ELISA and Western Blotting.
ELISA Analysis: A representative lot detected EWS in ELISA applications (Suarez-Calvet, M, et. al. (2016). Acta Neropathol. 131(4):587-604).
Western Blotting Analysis: A representative lot detected EWS in Western Blotting applications (Suarez-Calvet, M, et. al. (2016). Acta Neropathol. 131(4):587-604).
Research Category
Epigenetics & Nuclear Function
Quality
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected EWS in HeLa cell lysate.
Target description
~85 kDa observed; 68.48 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physical form
Purified rat monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G purified
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rat
antibody form	purified immunoglobulin
antibody product type	primary antibodies
clone	21B1, monoclonal
species reactivity	human
packaging	antibody small pack of 25 µL
application(s)	ELISA: suitable","western blot: suitable
isotype	IgG1
NCBI accession no.	NP_053733,
UniProt accession no.	Q01844,

WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable