Anti-Estrogen Receptor Antibody, clone 60C, rabbit monoclonal

eCl@ss:	32160702
NACRES:	NA.41

Кат. номер

4-820-25UL

4-820

4-820-100UL

The Estrogen Receptors (ER) belong to the nuclear receptor family that contains a ligand-binding domain that maintains the protein in an inactive conformation unless bound to the hormone. Two ERs are known, ERα, classically known as ER, and ERβ, described long after ERα. Activated Estrogen Receptor is a DNA-binding protein capable of inducing many changes in gene expression. The Estrogen Receptor is believed to play an important role in the growth and development of a subset of hormone dependent human breast cancers. ERβ is expressed in prostate, ovary, and other reproductive organs. Although its steroid specificity is very similar to that of the ERα, the molecular mechanisms regulating the transcriptional activity of ERβ may be distinct from those regulating ERα.
Specificity
Recognizes Estrogen Receptor α. No cross-reactivity with Estrogen Receptor β.
Predicted to cross-react with rat, mouse, bovine and porcine based on sequence homology.
Epitope: N-term
KLH-conjugated, synthetic peptide corresponding to amino acids 13-32 (C-ALLHQIQGNELEPLNRPQLK) of human Estrogen Receptor α. The immunizing sequence is identical in rat and mouse and has 19/20 identical amino acids in bovine and porcine.
Anti-Estrogen Receptor α Antibody, clone 60C is an antibody against Estrogen Receptor α for use in WB & IP.
Research Sub Category
Transcription Factors
Immunoprecipitation:
4-10 µL of a previous lot of this clone immunoprecipitated Estrogen Receptor from 1 mg of MCF-7 RIPA lysate.
Research Category
Epigenetics & Nuclear Function
Quality
Routinely evaluated by western blot analysis.
Western Blot Analysis:
This lot detected ER at a 1:2,000 dilution in MCF-7 lysates via SDS-PAGE and transferred to PVDF (Immobilon-P).
Target description
66 kDa
Replaces: 05-820
Physical form
Unpurified
Cultured supernantant with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at -20°C from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit
Quality Level	100
antibody form	culture supernatant
antibody product type	primary antibodies
clone	60C, monoclonal
species reactivity	mouse, pig, bovine, rat, human
packaging	antibody small pack of 25 µL
application(s)	immunoprecipitation (IP): suitable","western blot: suitable
isotype	IgG
NCBI accession no.	NP_000116.2,
UniProt accession no.	P03372,
shipped in	dry ice