Anti-EPO-R Antibody, clone VP-2E8

eCl@ss:

32160702

Кат. номер

MABS1287

MABS1287-25UG

Erythropoietin receptor (UniProt: P19235, also known as EPO-R) is encoded by the EPOR gene (Gene ID: 2057) in human. EPO-R is a single-pass type I membrane protein that serves as a receptor for erythropoietin. It mediates erythropoietin-induced erythroblast proliferation and differentiation. Upon EPO stimulation, EPO-R dimerizes triggering the JAK2/STAT5 signaling cascade. EPO-R has an extracellular domain (aa 25-250), a transmembrane domain (aa 251-273), and a cytoplasmic domain (aa 274-508). It is synthesized with a signal peptide (aa 1-24) that is cleaved to generate mature EPO-R. Upon stimulation following Erythropoietin (EPO) binding, EPO-R is phosphorylated on C-terminal tyrosine residues by JAK2. The phosphotyrosine motifs are also recruitment sites for several SH2-containing proteins and adapter proteins, which mediate cell proliferation. Phosphorylation on Tyrosine 454 is shown to be essential for PTPN6 interaction, and on Tyrosine 426 for PTPN11 interaction. Tyrosine 426 is also required for SOCS3 binding, however, Tyr-454/Tyr-456 motif is reported to be the preferred binding site. Ubiquitination of EPO-R at Lysine 81 is required for receptor internalization, whereas ubiquitination at Lysine 453 promotes trafficking of activated receptors to the lysosomes for degradation. Defects in EPOR gene are linked to familial erythrocytosis which is characterized by increased serum red blood cell mass, elevated hemoglobin and hematocrit, and hypersensitivity of erythroid progenitors to erythropoietin.
Specificity
Clone VP-2E8 recognizes EPO-R in human cells. It targets an epitope within the extracellular domain.
A recombinant fragment corresponding to the extracellular domain of human EPO-R.
Epitope: extracellular domain
Research Category
Signaling
Flow Cytometry Analysis: 2 µg/mL from a representative lot detected EPO-R in UT-7 (EPO dependent) cells.
Immunoprecipitation Analysis: A representative lot detected EPO-R in Immunoprecipitation applications (Maxwell, P., et. al. (2015). Br J Haematol. 168(3):429-42).
Immunofluorescence Analysis: A representative lot detected EPO-R in Immunofluorescence applications (Maxwell, P., et. al. (2015). Br J Haematol. 168(3):429-42).
Flow Cytometry Analysis: A representative lot detected EPO-R in Flow Cytometry applications (Maxwell, P., et. al. (2015). Br J Haematol. 168(3):429-42).
Anti-EPO-R, clone VP-2E8, Cat. No. MABS1287, is a mouse monoclonal antibody that detects erythropoietin receptor and is tested for use in Flow Cytometry, Immunocytochemistry, Immunofluorescence, and Immunoprecipitation.
Quality
Evaluated by Immunocytochemistry in starved UT-7 cells.
Immunocytochemistry Analysis: 1:500 dilution of this antibody detected EPO-R in starved UT-7 cells.
Target description
55.06 kDa calculated.
Physical form
Format: Purified
Purified mouse monoclonal antibody IgG1 in PBS without preservatives.
Protein G purified
Storage and Stability
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality Level	100
biological source	mouse
antibody form	purified immunoglobulin
antibody product type	primary antibodies
clone	VP-2E8, monoclonal
species reactivity	human
packaging	antibody small pack of 25 µg
application(s)	flow cytometry: suitable","immunocytochemistry: suitable","immunofluorescence: suitable","immunoprecipitation (IP): suitable
isotype	IgG1
NCBI accession no.	NP_000112,
UniProt accession no.	P19235,
shipped in	ambient