Anti-Epidermal Growth Factor (EGF) Receptor antibody, Mouse monoclonal

NACRES:

NA.41

Кат. номер

SAB4200802-25UL

SAB4200802-100UL

Epidermal growth factor receptor (EGFR), also known as Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1 or HER-1, is an integral cell membrane protein which spans the membranes of a wide range of normal and malignant epithelial cells. EGFR is a tyrosine-specific protein kinase domain, it has an extracellular region which binds EGF and an intra-cellular carboxy-terminal region which possesses the tyrosine kinase activity.
Monoclonal Anti-Epidermal Growth Factor (EGF) Receptor (mouse IgG1 isotype) is derived from the F4 hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from a mouse.
peptide corresponding to the C-terminal region of human EGF receptor (EGFR) (GeneID 1956), coupled to KLH
Anti-Epidermal Growth Factor (EGF) Receptor antibody, Mouse monoclonal has been used in:

• immunoblotting
• immunoprecipitation
• immunohistochemistry
• immunofluorescence

Monoclonal Anti-Epidermal Growth Factor (EGF) Receptor recognizes intracellular domain of the EGF receptor. The antibody is recommended to use in various immunological techniques, including Immunobloting (∼170 kDa), Immunoprecipitation, Immunohistochemistry and Immunofluorescence.
Biochem/physiol Actions
Epidermal growth factor (EGF) binding to its specific EGFR, results in an increased DNA synthesis initiating a signaling cascades including cell proliferation, differentiation and repair of damaged epithelial tissue. The EGFR has a half-life of approximately ~10 hours in human fibroblasts, but in the presence of EGF this value is reduced to about 1hour. A close similarity has been found between the sequence of the v-erb-B oncogene and the cytoplasmic transmembrane domain of the EGFR (truncated EGFR). Overexpression or mutation of EGFR may enhance DNA repair mechanisms, thereby contributing to radio- and chemotherapy resistance. High levels of EGFR have been identified in sarcomas, gliomas, gynecological, breast, bladder and lung tumors.
Physical form
Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative.
This product is for R&D use only, not for drug, household, or other uses.

biological source	mouse
antibody form	purified from hybridoma cell culture
antibody product type	primary antibodies
clone	F4, monoclonal
form	buffered aqueous solution
mol wt	antigen ~170 kDa
species reactivity	human
packaging	antibody small pack of 25 µL
concentration	~1.0 mg/mL
application(s)	immunoblotting: 0.3-0.6 µg/mL using human epidermoid carcinoma A431 cell line extract","immunofluorescence: suitable","immunohistochemistry: suitable","immunoprecipitation (IP): suitable
isotype	IgG1
UniProt accession no.	P00533,
shipped in	dry ice
storage temp.	20°C
Gene Information	human ... EGFR(1956)

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable