Anti-EBNA-1 Antibody, clone 1EB12

Кат. номер

MABE1881-25UL

MABE1881-100UL

Epstein-Barr nuclear antigen 1 (UniProt: P03211; also known as EBNA-1, EBV nuclear antigen 1) is encoded by the EBNA1 (also known as BKRF1) gene (Gene ID: 3783709) in Epstein-Barr virus (EBV). EBV is a gamma-herpesvirus, a causative agent of infectious mononucleosis. EBNA1, a multi-functional protein, plays an important role in maintaining EBV in the host cell and has been implicated in host cell immortalization. It plays an essential role in replication and partitioning of viral genomic DNA during latent viral infection. During this phase, the circular double-stranded viral DNA undergoes replication once per cell cycle and is efficiently partitioned to the daughter cells. EBNA1 activates the initiation of viral DNA replication through binding to specific sites in the viral latent origin of replication, oriP. It is also reported to direct the segregation of viral episomes by mediating their attachment to host cell metaphase chromosomes and is known to activate the transcription of several viral latency genes. By counteracting the stabilization of host p53, it also reduces apoptosis and increases host cell survival. EBNA1 is also a risk factor in a variety of malignancies and is the only viral protein found in all EBV-related malignancies. Its C-terminal region contains a dimerization domain and a DNA binding domain (aa 459-607). Although this domain is essential for its replication function of EBNA1, it is not sufficient for this activity and the N-terminal half of EBNA1 is also required. (Ref.: Sun, X., et al. (2010). Proc. Natl. Acad. Sci. USA. 107(7); 3146-3151; Duellman, SJ., and Burgess, RR. (2009). PLoS ONE 4(2): e4614; Wu, H., et al. (2002). J. Virol. 76(5); 2480-2490).
Specificity
Clone 1EB12 is a mouse monoclonal antibody that detects Epstein-Barr nuclear antigen 1 (EBNA-1). It targets an epitope within 26 amino acids from the N-terminal region.
His-tagged recombinant Epstein-Barr nuclear antigen 1 expressed in E. coli.
Quality Control Testing
Evaluated by Western Blotting in Raji cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected EBNA-1 in Raji cell lysate.
Tested Applications
Immunoprecipitation Analysis: A representative lot immunoprecipitated EBNA-1 in Immunoprecipitation application. (Duellman, S.J., et al. (2009). PLoS One. 4(2):e4614; Sun, X., et al. (2010). Proc Natl Acad Sci USA. 107(7):3146-51).
ELISA Analysis: A representative lot detected EBNA-1 in ELISA application. (Duellman, S.J., et al. (2009). PLoS One. 4(2):e4614).
Western Blotting Analysis: A representative lot detected EBNA-1 in Western Blotting application. (Duellman, S.J., et al. (2009). PLoS One. 4(2):e4614; Sun, X., et al. (2010). Proc Natl Acad Sci USA. 107(7):3146-51).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-EBNA-1, clone 1EB12, Cat. No. MABE1881, is a mouse monoclonal antibody that detects Epstein-Barr nuclear antigen 1 and is tested for use in ELISA, Immunoprecipitation, and Western Blotting.
Physical form
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality Level	200
biological source	mouse
antibody form	purified antibody
antibody product type	primary antibodies
clone	1EB12, monoclonal
mol wt	calculated mol wt 56.43 kDa","observed mol wt ~56 kDa
purified by	using protein G
species reactivity	virus
packaging	antibody small pack of 100 µL
application(s)	ELISA: suitable","immunoprecipitation (IP): suitable","western blot: suitable
isotype	IgG1
epitope sequence	N-terminal
conjugate	unconjugated
UniProt accession no.	P03211,
shipped in	2-8°C
Gene Information	vaccinia virus ... EBNA-1(3783774)

WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable