- Производитель:
- Sigma-Aldrich
eCl@ss: | 32160702 |
NACRES: | NA.41 |
Кат. номер |
ABE2587 |
ABE2587-25UL |
Specificity
This rabbit polyclonal antibody detects Dyskerin in human cells. It targets a 16 amino acid epitope within the C-terminal half of the Dyskerin protein. For immunoprecipitaion the use of HEPES or Tris bufer with KCl is highly recommended.
Epitope: unknown
Epigenetics & Nuclear Function
Anti-Dyskerin (Rabbit), Cat. No. ABE2587, is a highly specific rabbit polyclonal antibody that targets H/ACA ribonucleoprotein complex subunit 4 and has been tested for use in Immunofluorescence, Immunoprecipitation, and in Western Blotting.
Western Blotting Analysis: A 1:500 dilution from a representative lot detected Dyskerin in 10 µg of HeLa cell lysate.
Immunoprecipitation Analysis: A representative lot recovered Dyskerin associated with active telomerase in telomerase-overexpressing HEK293T cells (Courtesy of the laboratories of Dr. Hilda Pickett and Dr. Tracy Bryan, Children s Medical Research Institute, Australia).
Western Blotting Analysis: A representative lot detected Dyskerin in Western Blotting applications (OBrien, R., et. al. (2016). Cancer Res. 76(12):3604-17).
Western Blotting Analysis: A representative lot detected Dyskerin in mid-S phase HEK293T cells. The signal depleted upon treatment with siRNA against Dyskerin. (Moye, A.L., et. al. (2015). Nat Commun. 6:7643).
Immunofluorescence Analysis: A representative lot detected Dyskerin in HeLa cells. (telomerase positive) (Courtesy of the laboratories of Dr. Hilda Pickett and Dr. Tracy Bryan, Children s Medical Research Institute, Australia).
Western Blotting Analysis: A representative lot detected Dyskerin in HEK293 Cell lysate. The signal was depleted upon treatment with siRNA against Dyskerin (Courtesy of the laboratories of Dr. Hilda Pickett and Dr. Tracy Bryan, Children s Medical Research Institute, Australia).
Quality
Evaluated by Western Blotting in SW480 cell lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected Dyskerin in 10 µg of SW480 cell lysate.
Target description
~58 kDa observed; 57.67 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physical form
Purified rabbit polyclonal antibody in buffer containing 20 mM Tris, 200 mM KCl, 1 mM EDTA, pH 8.5 with 0.02% sodium azide.
Affinity Purified
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
biological source | rabbit |
Quality Level | 100 |
antibody form | affinity isolated antibody |
antibody product type | primary antibodies |
clone | polyclonal |
purified by | affinity chromatography |
species reactivity | human |
packaging | antibody small pack of 25 µL |
application(s) | immunofluorescence: suitable","immunoprecipitation (IP): suitable","western blot: suitable |
isotype | IgG |
UniProt accession no. | O60832, |
WGK Germany | nwg |
Flash Point F | Not applicable |
Flash Point C | Not applicable |
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