- Производитель:
- Sigma-Aldrich
eCl@ss: | 32160702 |
NACRES: | NA.41 |
Кат. номер |
ABS1644 |
ABS1644-25UL |
Specificity
This rabbit polyclonal antibody detects DsbA-L (Glutathione S-transferase kappa 1) in human and mouse cells.
Epitope: unknown
Western Blotting Analysis: A representative lot detected DsbA-L in mouse brain, mouse kidney, and mouse liver (Liu, M., et. al. (2008). Proc Natl Acad Sci USA. 105(47):18302-7; Lui, M., et. al. (2015). J Biol Chem. 290(16):10143-8).
Immunoprecipitation Analysis: A representative lot detected DsbA-L in 3T3-L1 adipocytes (Liu, M., et. al. (2008). Proc Natl Acad Sci USA. 105(47):18302-7; Lui, M., et. al. (2015). J Biol Chem. 290(16):10143-8).
Immunocytochemistry Analysis: A representative lot detected DsbA-L in adipocytes on differentiation day 7 were used for the following studies. confocal immunofluorescence study of DsbA-L co-localization with the mitochondrial marker (MitoTracker) and the ER marker (PDI) in 3T3-L1 adipocytes (Lui, M., et. al. (2015). J Biol Chem. 290(16):10143-8).
Research Category
Signaling
Anti-DsbA-L, Cat. No. ABS1644, is a rabbit polyclonal antibody that detects Glutathione S-transferase kappa 1 (DsbA-L) and is tested for use in Immunocytochemistry, Immunoprecipitation, and Western Blotting.
Quality
Evaluated by Western Blotting in mouse kidney tissue lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected DsbA-L in 10 µg of mouse kidney tissue lysate.
Target description
~28 kDa observed; 25.70 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physical form
Format: Unpurified
Rabbit polyclonal antiserum with 0.05% sodium azide with 20% glycerol.
Unpurified
Rabbit polyclonal antiserum with 0.05% sodium azide with 20% glycerol.
Storage and Stability
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
biological source | rabbit |
Quality Level | 100 |
antibody form | unpurified |
antibody product type | primary antibodies |
clone | polyclonal |
species reactivity | mouse, human |
packaging | antibody small pack of 25 µL |
application(s) | immunocytochemistry: suitable","immunoprecipitation (IP): suitable","western blot: suitable |
isotype | IgG |
NCBI accession no. | NP_083831, |
UniProt accession no. | Q9DCM2, |
shipped in | ambient |
Flash Point F | does not flash |
Flash Point C | does not flash |
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