Anti-DOCK9 (Zizimin 1) antibody, Mouse monoclonal

NACRES:

NA.41

Кат. номер

SAB4200093-25UL

SAB4200093-200UL

Monoclonal Anti-DOCK9 (Zizimin1) (mouse IgG2a isotype) is derived from the hybridoma 8B3-C3-E4 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. Zizimin1 is a guanine nucleotide exchange factor (GEF) specific to Cdc42. It is a part of the second family of mammalian Rho-GEFs, CZH [CDM (Ced-5/DOCK180/Myoblast city)-zizimin homology] proteins. The Zizimin1 protein contains the CZH2 domain, CZH1 domain and Pleckstrin homology (PH) domain.
Specificity
Monoclonal Anti-DOCK9 (Zizimin1) recognizes human DOCK9.
full length DOCK9 (Zizimin1)
Anti-DOCK9 (Zizimin 1) antibody, Mouse monoclonal is suitable for:

• immunoblotting
• immunoprecipitation
• immunocytochemistry
• immunohistochemistry

Biochem/physiol Actions
Zizimin1/ dedicator of cytokinesis 9 (DOCK9) is found to activate Cdc42 through binding to its CZH2 domain. Interestingly, the CZH1 domain of Zizimin1 at the N-terminal binds to its CZH2 domain and thus inhibits Cdc42 binding. The Pleckstrin homology (PH) domain at the N-terminal, binds phosphoinositides and results in zizimin1 membrane targeting.
Physical form
Solution in 0.01M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.
Storage and Stability
Store at -20 °C. For continuous use, the product may be stored at 2-8 °C for up to one month. For extended storage, freeze in working aliquots at -20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	mouse
antibody form	purified from hybridoma cell culture
antibody product type	primary antibodies
clone	8B3-C3-E4, monoclonal
form	buffered aqueous solution
mol wt	antigen ~236 kDa
species reactivity	human
packaging	antibody small pack of 25 µL
concentration	~1.0 mg/mL
application(s)	immunocytochemistry: suitable","immunohistochemistry: suitable","immunoprecipitation (IP): suitable","western blot: 3.0-6.0 µg/mL using MDA-MB-231 cell extracts
conjugate	unconjugated
UniProt accession no.	Q9BZ29,
shipped in	dry ice
storage temp.	20°C
Gene Information	human ... DOCK9(23348)

RIDADR	NONH for all modes of transport
Flash Point F	Not applicable
Flash Point C	Not applicable